You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Anti-Pyruvate Dehydrogenase E1 beta subunit antibody [17A5E2H8 ] (ab110331)

MSCatalog No. MSP04

CodeSizePriceAbpointsAvailability
    
Add to basket
 

  • -

  •   
  •   
  •   

  •  

  •  
  •  

  •  

  •  
Updating...

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »

Overview

Product name

Anti-Pyruvate Dehydrogenase E1 beta subunit antibody [17A5E2H8 ]
See all Pyruvate Dehydrogenase E1 beta subunit products (4) ...

Description

Mouse monoclonal [17A5E2H8 ] to Pyruvate Dehydrogenase E1 beta subunit

Tested applications

WBmore details

Cross reactivity

Reacts with

Mouse, Rat, Cow, Human

Immunogen

Porcine Pyruvate Dehydrogenase E1 beta subunit protein.

Positive control

Isolated mitochondria from Human, Bovine, Rat and Mouse hearts; HepG2 cell lysate.

Properties

Form

Liquid

Storage instructions

Store at +4°C. Do not freeze.

Storage buffer

Preservative: 0.02% Sodium azide
Constituent: HBS

Concentration

Concentration information loading...

Purity

IgG fraction

Purification notes

ab110331 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.

Clonality

Monoclonal

Clone number

17A5E2H8

Isotype

IgG1

Light chain type

kappa

Research areas

Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Energy transfer pathways >> Energy Metabolism
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Carbohydrate metabolism
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Signal Transduction >> Metabolism >> Mitochondrial
Signal Transduction >> Metabolism >> Energy Metabolism
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria

  • Western blot - Pyruvate Dehydrogenase E1 beta subunit antibody [17A5E2H8 ] (ab110331)Western blot - Pyruvate Dehydrogenase E1 beta subunit antibody [17A5E2H8 ] (ab110331) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab110331 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).

Involvement in disease

Defects in PDHB are a cause of pyruvate dehydrogenase E1 component deficiency (PDHE1 deficiency) [MIM:312170]. PDHE1 deficiency is the most common enzyme defect in patients with primary lactic acidosis. It is associated with variable clinical phenotypes ranging from neonatal death to prolonged survival complicated by developmental delay, seizures, ataxia, apnea, and in some cases to an X-linked form of Leigh syndrome (LS) (Leigh encephalomyelopathy).

Cellular localization

Mitochondrion matrix.

Target information above from: UniProt accessionP11177 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • mitochondrial antibody
  • DKFZp564K0164 antibody
  • MSP04 antibody
  • ODPB_HUMAN antibody
  • pdhB antibody
  • PDHE1 B antibody
  • PDHE1-B antibody
  • PHE1B antibody
  • Pyruvate dehydrogenase (lipoamide) beta antibody
  • Pyruvate dehydrogenase E1 beta polypeptide antibody
  • Pyruvate dehydrogenase E1 component subunit beta antibody
  • Pyruvate dehydrogenase E1 component subunit beta mitochondrial antibody
see all

Database links

see all

Anti-Pyruvate Dehydrogenase E1 beta subunit antibody [17A5E2H8 ] images:

  Western blot - Pyruvate Dehydrogenase E1 beta subunit antibody [17A5E2H8 ] (ab110331)

Western blot - Pyruvate Dehydrogenase E1 beta subunit antibody [17A5E2H8 ] (ab110331)

All lanes : Anti-Pyruvate Dehydrogenase E1 beta subunit antibody [17A5E2H8 ] (ab110331) at 1 µg/ml

Lane 1 : Isolated mitochondria from Human heart at 10 µg/ml
Lane 2 : Isolated mitochondria from Bovine heart at 4 µg/ml
Lane 3 : Isolated mitochondria from Rat heart at 10 µg/ml
Lane 4 : Isolated mitochondria from Mouse heart at 10 µg/ml
Lane 5 : HepG2 cell lysate at 20 µg/ml


Predicted band size : 39 kDa

References for Anti-Pyruvate Dehydrogenase E1 beta subunit antibody [17A5E2H8 ] (ab110331)

This product has been referenced in:

  • Halim ND  et al. Phosphorylation status of pyruvate dehydrogenase distinguishes metabolic phenotypes of cultured rat brain astrocytes and neurons. Glia 58:1168-76 (2010).Read more (PubMed: 20544852) »
  • Han Z  et al. A combined therapeutic approach for pyruvate dehydrogenase deficiency using self-complementary adeno-associated virus serotype-specific vectors and dichloroacetate. Mol Genet Metab 93:381-7 (2008).Read more (PubMed: 18206410) »

See all 3 publications for this product

Publishing research using ab110331? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"