![Immunoprecipitation - Pyruvate Dehydrogenase Immunocapture antibody [15D3G9C11] (ab109866) image](#Pyruvate-Dehydrogenase-Immunocapture-Primary-antibodies-ab109866-1.jpg)
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Product name
Anti-Pyruvate Dehydrogenase Immunocapture antibody [15D3G9C11]
Description
Mouse monoclonal [15D3G9C11] to Pyruvate Dehydrogenase Immunocapture
Specificity
100 µg ab109866 can capture at least 5 µg Pyruvate Dehydrogenase complex from 1 mg solubilized Bovine heart mitochondria.
Tested applications
Cross reactivity
Reacts with
Cow, Human
Predicted to work with
Pig
Immunogen
Pig heart Pyruvate Dehydrogenase
Positive control
Bovine heart mitochondria
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: HBS
Concentration
Concentration information loading...
Purification notes
ab109866 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Clonality
Monoclonal
Clone number
15D3G9C11
Isotype
IgG1
Light chain type
kappa
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Signal Transduction >> Metabolism >> Mitochondrial
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Immunoprecipitation - Pyruvate Dehydrogenase Immunocapture antibody [15D3G9C11] (ab109866)
![Immunoprecipitation - Pyruvate Dehydrogenase Immunocapture antibody [15D3G9C11] (ab109866) image](/ps/datasheet/images/109/ab109866/Pyruvate-Dehydrogenase-Immunocapture-Primary-antibodies-ab109866-1.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab109866 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at 100-1 µg/mg of lysate.
Application notes
Is unsuitable for ,IHC-P or WB.
Target
Relevance
The pyruvate dehydrogenase complex (PDH) is at the center of aerobic carbohydrate metabolism. It is localized in the matrix space of mitochondria where it catalyzes the irreversible oxidative decarboxylation of pyruvate entering the organelle to produce acetyl-CoA, NADH and CO2. PDH is a complex of three different enzymes that channel substrate and product. These are pyruvate dehydrogenase (E1), dihydrolipoamide transacetylase (E2) and dihydrolipoamide dehydrogenase (E3). PDH deficiencies, diabetes, starvation, sepsis, and possibly Alzheimer's disease have been linked to altered PDH functioning.
Cellular localization
Mitochondrial
Anti-Pyruvate Dehydrogenase Immunocapture antibody [15D3G9C11] images:
Immunoprecipitation - Pyruvate Dehydrogenase Immunocapture antibody [15D3G9C11] (ab109866)
Immunoprecipitation of Pyruvate Dehydrogenase using ab109866 at 100 µg/mg lysate.
References for Anti-Pyruvate Dehydrogenase Immunocapture antibody [15D3G9C11] (ab109866)
This product has been referenced in:
- Murray J et al. Focused proteomics: towards a high throughput monoclonal antibody-based resolution of proteins for diagnosis of mitochondrial diseases. Biochim Biophys Acta 1659:206-11 (2004).Read more (PubMed: 15576053) »
See 1 publication for this product
Publishing research using ab109866? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"