Pyruvate dehydrogenase (PDH) WB Antibody Cocktail (ab110416)
Key features and details
- Assay type: Quantitative
Overview
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Product name
Pyruvate dehydrogenase (PDH) WB Antibody Cocktail -
Assay type
Quantitative -
Species reactivity
Reacts with: Cow, Human -
Product overview
ab110416 (MSP02) contains a total of 150 µg of monoclonal antibodies reactive against PDH subunits. Isolated mitochondria are available from several species for use as control samples.
The PDH Western blotting kit contains four different mAbs reacting specifically with E1 alpha, E1 beta, E2 and E2/E3bp. A mAb against the OSCP subunit of ATP synthase is included as a control. This mAb mixture can be used to probe the isolated pyruvate dehydrogenase for integrity and purity.
Cocktail Antibodies:
Mouse anti C-V-OSCP [4C11C10D12] monoclonal:
Amount: 12.5 µg
Working concentration: 0.5 µg/mlMouse anti PDH-E1-alpha [9H9AF5] monoclonal:
Amount: 25 µg
Working concentration: 1 µg/mlMouse anti PDH-E1-beta [17A5E2H8] monoclonal:
Amount: 62.5 µg
Working concentration: 2.5 µg/mlMouse anti PDH-E2 [15D3G9C11] monoclonal:
Amount: 25 µg
Working concentration: 1 µg/mlMouse anti PDH-E2/E3bp [13G2AE2BH5] monoclonal:
Amount: 25 µg
Working concentration: 1 µg/ml -
Tested applications
Suitable for: WBmore details
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 150 µg 150 µg Cocktail of 5 Antibodies 1 x 150µg 1 x 150µg -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110416 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 6 µg/ml. Suggested concentration.
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Notes |
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WB
Use a concentration of 6 µg/ml. Suggested concentration. |
Images
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Blocked for overnight with 5% Milk/PBS at 4ºC (or 3 hrs at room temperature).
Primary antibody incubatedat 6 µg/mL in 1% milk/PBS for 2hrs at room temperature.
Secondary: HRP-conjugated anti-mouse used according to manufacturers instructions.
Detection method: ECL+
All wash steps carried out in PBS + 0.05% Tween-20.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (30)
ab110416 has been referenced in 30 publications.
- Sugiura K et al. MuRF1 deficiency prevents age-related fat weight gain, possibly through accumulation of PDK4 in skeletal muscle mitochondria in older mice. J Orthop Res 40:1026-1038 (2022). PubMed: 34185335
- Hegazy L et al. Identification of Novel Mitochondrial Pyruvate Carrier Inhibitors by Homology Modeling and Pharmacophore-Based Virtual Screening. Biomedicines 10:N/A (2022). PubMed: 35203575
- De Rosa V et al. Non-Canonical Role of PDK1 as a Negative Regulator of Apoptosis through Macromolecular Complexes Assembly at the ER-Mitochondria Interface in Oncogene-Driven NSCLC. Cancers (Basel) 13:N/A (2021). PubMed: 34439291
- Chambers KT et al. Myocardial Lipin 1 knockout in mice approximates cardiac effects of human LPIN1 mutations. JCI Insight 6:N/A (2021). PubMed: 33986192
- Kaya E et al. Beneficial Effects of Acetyl-DL-Leucine (ADLL) in a Mouse Model of Sandhoff Disease. J Clin Med 9:N/A (2020). PubMed: 32276303