Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibody (ab86963)

Overview

• Product nameAnti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibodySee all Mitochondrial Pyruvate dehydrogenase kinase 1 primary antibodies ...
• Description
  Rabbit polyclonal to Mitochondrial Pyruvate dehydrogenase kinase 1
• SpecificityDetects isoform 1.
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat, Cow
• Immunogen

  Synthetic peptide corresponding to a region within the internal sequence amino acids 288-337 (TMEHHANRGV YPPIQVHVTL GNEDLTVKMS DRGGGVPLRK IDRLFNYMYS) of Human PDK1, NP_002601

  Run BLAST with Run BLAST with
• Positive controlMCF7 cell lysate

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: 2% Sucrose, PBS
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Energy transfer pathways
  • Integration of energy

  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Energy transfer pathways
  • Energy Metabolism

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Carbohydrate metabolism

  • Cancer
  • Cancer Metabolism
  • Metabolic signaling pathway
  • Metabolism of carbohydrates

  • Cancer
  • Cancer Metabolism
  • Metabolic signaling pathway
  • Integration of energy metabolism

  • Cancer
  • Cancer Metabolism
  • Cellular metabolic process

  • Signal Transduction
  • Metabolism
  • Energy Metabolism

  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • Other Kinases

  • Signal Transduction
  • Cytoskeleton / ECM
  • Cytoskeleton
  • Microfilaments
  • Actin etc
  • Actin Assembly

Applications

Target

• FunctionInhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
• Tissue specificityExpressed predominantly in the heart.
• Sequence similaritiesBelongs to the PDK/BCKDK protein kinase family.
  Contains 1 histidine kinase domain.
• Cellular localizationMitochondrion matrix.

Target information above from: UniProt accession Q15118 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 528655 Cow
  • Entrez Gene: 5163 Human
  • Entrez Gene: 228026 Mouse
  • Entrez Gene: 116551 Rat
  • Omim: 602524 Human
  • SwissProt: Q15118 Human
  • SwissProt: Q8BFP9 Mouse
  • SwissProt: Q63065 Rat

  • Unigene: 470633 Human
  • Unigene: 470633 Human
  • Unigene: 34411 Mouse
  • Unigene: 11185 Rat

  see all

• Alternative names
  [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1 antibody[Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial antibodyHGNC:8809 antibody

  mitochondrial antibodyMitochondrial pyruvate dehydrogenase kinase isoenzyme 1 antibodyPdk1 antibodyPDK1_HUMAN antibodyPyruvate dehydrogenase kinase isoform 1 antibodyPyruvate dehydrogenase kinase, isoenzyme 1 antibody

  see all

Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibody images

• 

  Western blot - Pyruvate dehydrogenase kinase 1 antibody (ab86963)
  Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibody (ab86963) at 1 µg/ml + MCF7 cell lysate at 10 µg
  
  Secondary
  HRP conjugated anti-Rabbit IgG at 1/50000 dilution
  
  Predicted band size : 47 kDa
  Observed band size : 47 kDa