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Products:Neuroscience >> Neurology process >> Neural Signal Transduction
Overview
Product name
Anti-QK1 antibody
See all QK1 products (7) ...
Description
Goat polyclonal to QK1
Specificity
ab77176 is expected to recognise all reported isoforms of QK1.
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Mouse
Predicted to work with
Rat, Dog, Human, Pig, Xenopus laevis, Zebrafish
Immunogen
Synthetic peptide: ETKEKPKPTPDY-C, corresponding to N terminal amino acids 6-17 of Human QK1 (NP_006766.1; NP_996735.1; NP_996736.1; NP_996737.1)
ETKEKPKPTP DY-C
Positive control
Mouse brain lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab77176 is purified by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Splicing
Neuroscience >> Neurology process >> Neural Signal Transduction
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Western blot - QK1 antibody (ab77176)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab77176 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ELISA
Application notes
Peptide ELISA: Antibody detection limit dilution 1/64000.
WB: Use at a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 38 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
RNA-binding protein that plays a central role in myelinization. Binds to the 5'-NACUAAY-N(1,20)-UAAY-3' RNA core sequence. Acts by regulating pre-mRNA splicing, mRNA export, mRNA stability and protein translation. Required to protect and promote stability of mRNAs such as MBP and CDKN1B. Regulator of oligodendrocyte differentiation and maturation in the brain that may play a role in myelin and oligodendrocyte dysfunction in schizophrenia. Participates in mRNA transport by regulating the nuclear export of MBP mRNA. Also involved in regulation of mRNA splicing of MAG pre-mRNA. Acts as a translational repressor.
Tissue specificity
Expressed in the frontal cortex of brain. Down-regulated in the brain of schizophrenic patients.
Sequence similarities
Contains 1 KH domain.
Post-translational
modifications
Methylated by PRMT1.
Tyrosine phosphorylated at its C-terminus, probably by FYN. Phosphorylation leads to decreased mRNA-binding affinity, affecting transport and/or stabilization of MBP mRNA.
Cellular localization
Nucleus. Cytoplasm.
Target information above from: UniProt accessionQ96PU8
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DKFZp586I0923 antibody
- HKQ antibody
- Homolog of mouse quaking QKI KH domain RNA binding protein antibody
see all
Database links
- Entrez Gene: 606754 Dog
- Entrez Gene: 9444 Human
- Entrez Gene: 19317 Mouse
- Entrez Gene: 499022 Rat
- Omim: 609590 Human
- SwissProt: Q9GMY1 Dog
- SwissProt: Q96PU8 Human
- SwissProt: Q9QYS9 Mouse
see all
Anti-QK1 antibody images:
Western blot - QK1 antibody (ab77176)
Anti-QK1 antibody (ab77176) at 0.3 µg/ml + mouse brain lysate in RIPA buffer at 35 µg
Predicted band size : 38 kDa
Observed band size : 38 kDa
Primary incubation was 1 hour. Detected by chemiluminescence.
References for Anti-QK1 antibody (ab77176)
ab77176 has not yet been referenced specifically in any publications.
Publishing research using ab77176? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"