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Western blot - QK1 antibody (ab77176)
Western blot - QK1 antibody (ab77176)
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Product Name 

QK1 antibody

 

See all QK1 antibodies (4)...

Product type 

Primary antibodies

Description 

Goat polyclonal to QK1

Immunogen 

Synthetic peptide: ETKEKPKPTPDY-C, corresponding to N terminal amino acids 6-17 of Human QK1 (NP_006766.1; NP_996735.1; NP_996736.1; NP_996737.1)
BLAST 'ETKEKPKPTPDY-C' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Reacts with 

Ms

Predicted to react 
(species key)

Hu, Rat, Dog, Pig, Xl, Zfsh
(Due to sequence homology)

Specificity 

ab77176 is expected to recognise all reported isoforms of QK1.

Tested applications 
(see key)

ELISA, WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
Peptide ELISA: Antibody detection limit dilution 1/64000.
WB: Use at a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 38 kDa.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Mouse brain lysate.

Cellular localization 

Cytoplasmic and Nuclear (By similarity).

Research areas 

Chromatin and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Chromatin and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Splicing
Neuroscience >> Neurology process >> Neural Signal Transduction

Relevance 

The selective RNA binding protein QKI is essential for myelination in the central nervous system. QKI belongs to the family of signal transduction activators of RNA (STARs), characteristic of binding RNA and signaling molecules, therefore is postulated to regulate RNA homeostasis in response to developmental signals.

 

Alternative names QK1 antibody (ab77176)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

606754    (Dog)
9444    (Human)
19317    (Mouse)
499022    (Rat)

Omim

    

609590    (Human)

SwissProt

    

Q9GMY1    (Dog)
Q96PU8    (Human)
Q9QYS9    (Mouse)
Q5W9D5    (Pig)
Q91XU1    (Rat)

Raised in 

Goat

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

ab77176 is purified by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide.

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this QK1 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for QK1 antibody images, references, products related to ab77176 and other tools.

 

QK1 antibody images:

Product image  Western blot - QK1 antibody (ab77176)

Western blot - QK1 antibody (ab77176)

QK1 antibody (ab77176) at 0.3 µg/ml + mouse brain lysate in RIPA buffer at 35 µg

Predicted band size : 38 kDa
Observed band size : 38 kDa


Primary incubation was 1 hour. Detected by chemiluminescence.

 
 

Search PubMed (MEDLINE) for references to QK1

 

QK1 antibody - more information

Customer reviews (feedback) regarding QK1 antibody

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QK1 antibody - related products:

Products for Western blot

Loading controls

Lysates

 

Positive Controls for QK1 antibody

ab30148

  

Brain (Mouse) Cytoplasmic Lysate - normal tissue

ab30150

  

Brain (Mouse) Nuclear Lysate - normal tissue

 

Compatible Secondaries for QK1 antibody

ab6566

  

Cy5 ® antibody

ab6739

  

Texas Red ® antibody

ab6740

  

Biotin antibody

ab6741

  

HRP antibody

ab6742

  

Alkaline Phosphatase antibody

ab6881

  

FITC antibody

ab7004

  

Phycoerythrin antibody

ab27245

  

10nm Gold antibody

ab27246

  

10nm Gold antibody

ab27247

  

15nm Gold antibody

 

Other QK1 Antibodies

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QK1 antibody - other tools:

Contact Abcam with a Technical Enquiry about QK1 antibody

 

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