Recombinant Anti-QKI antibody [EPR7306] (ab126742)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7306] to QKI
- Suitable for: WB, IP, ICC/IF
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-QKI antibody [EPR7306]
See all QKI primary antibodies -
Description
Rabbit monoclonal [EPR7306] to QKI -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICC/IFmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human QKI aa 250 to the C-terminus. The exact sequence is proprietary.
Database link: Q96PU8 -
Positive control
- WB: HAP1, Neuro 2a, HeLa and K562 whole cell lysates. IP: HeLa whole cell lysate. ICC/IF: Neuro-2a cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Dissociation constant (KD)
KD = 4.30 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7306 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126742 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (2) |
1/1000 - 1/10000. Detects a band of approximately 38 kDa (predicted molecular weight: 37 kDa).
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IP |
1/10 - 1/100.
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ICC/IF |
1/50 - 1/100.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 38 kDa (predicted molecular weight: 37 kDa). |
IP
1/10 - 1/100. |
ICC/IF
1/50 - 1/100. |
Target
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Function
RNA-binding protein that plays a central role in myelinization (PubMed:16641098). Binds to the 5'-NACUAAY-N(1,20)-UAAY-3' RNA core sequence. Regulates target mRNA stability (PubMed:23630077). In addition, acts by regulating pre-mRNA splicing, mRNA export and protein translation. Required to protect and promote stability of mRNAs such as MBP and CDKN1B. Regulator of oligodendrocyte differentiation and maturation in the brain that may play a role in myelin and oligodendrocyte dysfunction in schizophrenia (PubMed:16641098). Participates in mRNA transport by regulating the nuclear export of MBP mRNA. Also involved in regulation of mRNA splicing of MAG pre-mRNA. Acts as a translational repressor. -
Tissue specificity
Expressed in the frontal cortex of brain. Down-regulated in the brain of schizophrenic patients. -
Sequence similarities
Contains 1 KH domain. -
Domain
The KH domain and the Qua2 region are involved in RNA binding. -
Post-translational
modificationsMethylated by PRMT1.
Tyrosine phosphorylated at its C-terminus, probably by FYN. Phosphorylation leads to decreased mRNA-binding affinity, affecting transport and/or stabilization of MBP mRNA. -
Cellular localization
Nucleus. Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 9444 Human
- Entrez Gene: 19317 Mouse
- Omim: 609590 Human
- SwissProt: Q96PU8 Human
- SwissProt: Q9QYS9 Mouse
- Unigene: 510324 Human
- Unigene: 130694 Mouse
- Unigene: 384135 Mouse
see all -
Alternative names
- DKFZp586I0923 antibody
- HKQ antibody
- Homolog of mouse quaking QKI KH domain RNA binding protein antibody
see all
Images
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All lanes : Anti-QKI antibody [EPR7306] (ab126742) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : QKI knockout HAP1 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : THP-1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 37 kDaLanes 1 - 4: Merged signal (red and green). Green - ab126742 observed at 37 kDa. Red - loading control, ab7291, observed at 50 kDa.
ab126742 was shown to specifically react with in wild-type HAP1 cells as signal was lost in QKI knockout cells. Wild-type and QKI knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. ab126742 and ab7291 (Mouse anti Tubulin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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Immunocytochemistry/ Immunofluorescence analysis of Neuro-2a (Mouse neuroblastoma neuroblast) cells labeling QKI with Purified ab126742 at 1:100 dilution (5.6μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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ab126742 (purified) at 1:30 dilution (2µg) immunoprecipitating QKI in HeLa whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab126742 & HeLa whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab126742 in HeLa whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
All lanes : Anti-QKI antibody [EPR7306] (ab126742) at 1/2000 dilution (purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 37 kDa
Blocking and diluting buffer : 5% NFDM/TBST -
All lanes : Anti-QKI antibody [EPR7306] (ab126742) at 1/1000 dilution (unpurified)
Lane 1 : Neuro 2a cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 37 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (16)
ab126742 has been referenced in 16 publications.
- Derbis M et al. Short antisense oligonucleotides alleviate the pleiotropic toxicity of RNA harboring expanded CGG repeats. Nat Commun 12:1265 (2021). PubMed: 33627639
- Chen X et al. QKI is a critical pre-mRNA alternative splicing regulator of cardiac myofibrillogenesis and contractile function. Nat Commun 12:89 (2021). PubMed: 33397958
- Xiong M et al. LncRNA DANCR represses Doxorubicin-induced apoptosis through stabilizing MALAT1 expression in colorectal cancer cells. Cell Death Dis 12:24 (2021). PubMed: 33414433
- Shen Y et al. G protein-coupled oestrogen receptor promotes cell growth of non-small cell lung cancer cells via YAP1/QKI/circNOTCH1/m6A methylated NOTCH1 signalling. J Cell Mol Med 25:284-296 (2021). PubMed: 33237585
- Konieczny P et al. Cyclic mismatch binding ligands interact with disease-associated CGG trinucleotide repeats in RNA and suppress their translation. Nucleic Acids Res 49:9479-9495 (2021). PubMed: 34358321