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Western blot - QPRT antibody (ab37515)
(enlarge)
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Product Name
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QPRT antibody
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See all QPRT antibodies (2)...
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Product type
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Primary antibodies
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Description
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Chicken polyclonal to QPRT
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Immunogen
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Synthetic peptide, corresponding to Internal sequence amino acids 123-237 of Human QPRT
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Reacts with
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Hu
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Tested applications
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WB
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Abreviews
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Application notes
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Recommended dilutions
WB: 1/2000. Detects a band of approximately 65 kDa (predicted molecular weight: 31 kDa). A fusion protein was used in this blot, hence the difference in M.Wt as compared to native protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Research areas
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Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Signal Transduction >> Metabolism >> Amino Acids
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Neuroscience >> Neurology process >> Neurodegenerative disease >> Huntington's disease
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Relevance
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QPRT is a key enzyme in catabolism of quinolinate, an intermediate in the tryptophan-nicotinamide adenine dinucleotide pathway. Quinolinate acts as a most potent endogenous exitotoxin to neurons. Elevation of quinolinate levels in the brain has been linked to the pathogenesis of neurodegenerative disorders such as epilepsy, Alzheimer's disease and Huntington's disease.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Chicken
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Clonality
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Polyclonal
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Isotype
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IgY
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: None
Constituents: PBS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this QPRT antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for QPRT antibody images, references, products related to ab37515 and other tools.
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QPRT antibody images:
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 Western blot - QPRT antibody (ab37515)
A fusion protein was used in this blot, hence the difference in molecular weight compared to native protein.
QPRT antibody (ab37515) at 1/2000 dilution + E. coli-derived fusion protein at 2.5 µg
Secondary
Goat anti-IgY-HRP at 1/1000 dilution
Predicted band size : 31 kDa
Observed band size : 65 kDa (why is the actual band size different from the predicted?)
Band size larger than expected as a fusion protein was used as positive control.
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Search PubMed (MEDLINE) for references to QPRT
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QPRT antibody - more information
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Customer reviews (feedback) regarding QPRT antibody |
Customer FAQs regarding QPRT antibody |
Protocols for QPRT antibody |
Price and availability of products related to QPRT antibody |
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QPRT antibody - related products:
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QPRT antibody - other tools:
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Contact Abcam with a Technical Enquiry about QPRT antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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