Anti-QRFP antibody (ab126388)
Key features and details
- Rabbit polyclonal to QRFP
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-QRFP antibody -
Description
Rabbit polyclonal to QRFP -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence: GREHAGCRFR FGRQDEGSEA TGFLPAAGEK TSGPLGNLAE ELNGYSRKKG GFSFR, corresponding to amino acids 78-132 of Human QRFP.
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Positive control
- WB: QRFP over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells. IHC-P: Human small intestine, prostate, pancreas and endometrium tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126388 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 15 kDa.
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Notes |
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 15 kDa. |
Target
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Function
Stimulates feeding behavior, metabolic rate and locomotor activity and increases blood pressure. May have orexigenic activity. May promote aldosterone secretion by the adrenal gland. -
Tissue specificity
Expressed widely in the brain with highest expression levels in the cerebellum, medulla, pituitary, retina, vestibular nucleus, and white matter. Also expressed in the bladder, colon, coronary artery, parathyroid gland, prostate, testis, and thyroid. -
Sequence similarities
Belongs to the RFamide neuropeptide family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 347148 Human
- Omim: 609795 Human
- SwissProt: P83859 Human
- Unigene: 660704 Human
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Alternative names
- 26RFa antibody
- Neuropeptide RF-amide antibody
- OX26_HUMAN antibody
see all
Images
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Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human small intestine tissue labeling QRFP with ab126388 at 1/500.
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Lane 3 : Anti-QRFP antibody (ab126388)
Lane 2 : Negative control (vector only transfected HEK293T lysate).
Lane 3 : Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY404880).
Predicted band size: 15 kDa
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY404880) -
Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human pancreas tissue labeling QRFP with ab126388 at 1/500.
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Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human endometrium tissue labeling QRFP with ab126388 at 1/500.
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Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human prostate tissue labeling QRFP with ab126388 at 1/500.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab126388 has not yet been referenced specifically in any publications.