Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 6 months.
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Our Abpromise guarantee covers the use of ab120247 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||FuncS: Use at an assay dependent concentration.|
ab13847 staining active caspase 3 in A549 cells treated with quercetin (ab120247), by ICC/IF. Increase in active caspase 3 expression correlates with increased concentration of quercetin, as described in literature.
The cells were incubated at 37°C for 6h in media containing different concentrations of ab120247 (quercetin) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab13847 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab968999) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
References for Quercetin (ab120247)
This product has been referenced in:
- Boots AW et al. Health effects of quercetin: from antioxidant to nutraceutical. Eur J Pharmacol 585:325-37 (2008). Read more (PubMed: 18417116) »
- Bischoff SC Quercetin: potentials in the prevention and therapy of disease. Curr Opin Clin Nutr Metab Care 11:733-40 (2008). Read more (PubMed: 18827577) »
- Formica JV & Regelson W Review of the biology of Quercetin and related bioflavonoids. Food Chem Toxicol 33:1061-80 (1995). Read more (PubMed: 8847003) »