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Quick Cell Proliferation Assay Kit II (ab65475)

Q&A (5)

Overview

  • Product nameQuick Cell Proliferation Assay Kit IISee all Cell proliferation kits ...
  • Tests
    500 x 1 assay
  • Sample type
    Adherent cells, Suspension cells
  • Assay typeQuantitative
  • Assay time
    4h 00m
  • Product overview

    Abcam's Quick Cell Proliferation Assay Kit II provides by far the easiest and most sensitive means for quantifying cell proliferation and viability. The assay is based on the cleavage of the tetrazolium salt to formazan by cellular mitochondrial dehydrogenase. The amount of the dye generated by activity of dehydrogenase is directly proportional to the number of living cells. The formazan dye produced by viable cells can be quantified by multi-well spectrophotometer (microtiter plate reader) by measuring the absorbance of the dye solution at 440 nm. The assay can be used for measurement of cell proliferation in response to growth factors, cytokines, mitogens, and nutrients, etc. It can also be used for the analysis of cytotoxic compounds like anticancer drugs and many other toxic agents and pharmaceutical compounds. The new method is so simple, just add-and-read, requiring no washing, no harvesting, and no solubilization steps. It is faster, stable, and more sensitive than MTT, XTT, MTS based assays. The assay correlates well with the [3H]-thymidine incorporation assay.
    Visit our FAQs page for tips and troubleshooting.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceCell proliferation is the multiplication or reproduction of cells, as a result of cell growth and cell division, resulting in the expansion of a cell population.

    • Applications

    Our Abpromise guarantee covers the use of ab65475 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS

    Protocols

    References for Quick Cell Proliferation Assay Kit II (ab65475)

    ab65475 has not yet been referenced specifically in any publications.

    Publishing research using ab65475 ? Please let us know so that we can cite the reference in this datasheet

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    Dear Sir/Madame,
    I would like to have more information about your two products: "Quick Cell Proliferation Assay Kit (ab65473)" and "Quick Cell Proliferation Assay Kit II (ab65475)"
    In particular I would ask if the serum in the ...

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    Thank you for contacting us and your interest in our products.

    The Quick Cell Proliferation Assay Kit (ab65473) and Quick Cell Proliferation Assay Kit II (ab65475) should not be affected by serum in your culture medium. Any differences from the d...

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    Inquiry: Hello, We are currently using a Quick cell proliferation assay kit II (#ab65475) in our lab and I would like to know the minimum sensitivity (ie OD) the kit can detect. Below which OD value can we consider the reading as non-relevant (even if we ...

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    Thank you for your enquiry and your interest in our products.

    As I understand you are looking for an absolute cut-off value for the OD. This is a comparative assay in which you can let the colour develop for an extended period of time to look at...

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    - The absorbance reading still going up even after 5 hours of incubation.

    Thank you for contacting us.
    I have now received an answer from lab. They say the colour might develop with longer incubation of the samples. However, the machine can detect the absorbance only within a certain limit. Keeping that in mind, we recomme...

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    The directions in the datasheet note that "Phenol Red in culture medium does not significantly interfere with the assay." But how will phenol red effect the assay and to what degree?

    The phenol red from the sample will be diluted in the reaction buffer to a degree that it will not interfere with the assay.

    Also, I am starting a new lab Univ of Missouri, Columbia. What sort of new lab start up promotions are available?

    Thank you for your enquiry. Currently our new lab promotion allows 20% off ALL orders plus FREE shipping on all orders for 3 months. The requirements to be considered a “New Lab” are the following: The lab is created by a scientist starting a completely n...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"