Quick Cell Proliferation Assay Kit II (ab65475)
- Product nameQuick Cell Proliferation Assay Kit IISee all Cell proliferation kits ...
- Tests500 x 1 assay
- Sample typeAdherent cells, Suspension cells
- Assay typeQuantitative
- Assay time4h 00m
- Product overview
Abcam's Quick Cell Proliferation Assay Kit II provides by far the easiest and most sensitive means for quantifying cell proliferation and viability. The assay is based on the cleavage of the tetrazolium salt to formazan by cellular mitochondrial dehydrogenase. The amount of the dye generated by activity of dehydrogenase is directly proportional to the number of living cells. The formazan dye produced by viable cells can be quantified by multi-well spectrophotometer (microtiter plate reader) by measuring the absorbance of the dye solution at 440 nm. The assay can be used for measurement of cell proliferation in response to growth factors, cytokines, mitogens, and nutrients, etc. It can also be used for the analysis of cytotoxic compounds like anticancer drugs and many other toxic agents and pharmaceutical compounds. The new method is so simple, just add-and-read, requiring no washing, no harvesting, and no solubilization steps. It is faster, stable, and more sensitive than MTT, XTT, MTS based assays. The assay correlates well with the [3H]-thymidine incorporation assay.
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- Tested applicationsFunctional Studies more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components 500 tests 2500 tests Electro Coupling Solution (ECS) 1 x 5ml 1 x 25ml Stop Solution 1 x 5ml 1 x 25ml WST Reagent (lyophilized) 1 vial 1 vial
- Research Areas
Our Abpromise guarantee covers the use of ab65475 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Quick Cell Proliferation Assay Kit II (ab65475)
ab65475 has not yet been referenced specifically in any publications.