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Products:Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab32004? Please let us know so that we can cite the reference in this datasheet
ab32004 has been referenced in 1 publications.
- Oehlke O et al. Rab11b and its effector Rip11 regulate the acidosis-induced traffic of V-ATPase in salivary ducts. J Cell Physiol 226:638-51 (2011). WB, ICC/IF; Human. PubMed: 20717956
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
![Immunohistochemistry (Paraffin-embedded sections) - Rab25 antibody [3F12F3] (ab32004)](/ps/datasheet/Images/32/ab32004/ab32004b.jpg)
Immunohistochemistry (Paraffin-embedded sections) - Rab25 antibody [3F12F3] (ab32004)
Immunohistochemisty analysis of parafin embedded carcinoma tissues, using ab32004. The staining shows the cytoplasmic location of Rab25. A DAB staining procedure was used to stain tissue sections.
![Western blot - Rab25 antibody [3F12F3] (ab32004)](/ps/datasheet/Images/32/ab32004/Image2.gif)
Western blot - Rab25 antibody [3F12F3] (ab32004)
All lanes : Anti-Rab25 antibody [3F12F3] (ab32004) at 1/500 dilution
Lane 1 : Human truncated Rab25 recombinant protein
Lane 2 : Ovary Carcinoma tissue lysate
Lane 3 : Stomach Carcinoma tissue lysate
Lane 4 : Breast Carcinoma tissue lysate
Predicted band size : 24 kDa
Observed band size : 28 kDa (why is the actual band size different from the predicted?)
![Immunocytochemistry/ Immunofluorescence - Rab25 antibody [3F12F3] (ab32004)](/ps/datasheet/images/32/ab32004/Rab25-Primary-antibodies-ab32004-1.jpg)
Immunocytochemistry/ Immunofluorescence - Rab25 antibody [3F12F3] (ab32004)
ICC/IF image of ab32004 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab32004, 5µg/ml) overnight at +4ºC. The secondary antibody (green) was ab97899 Dylight 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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