![Immunohistochemistry (Paraffin-embedded sections) - Rab25 antibody [3F12F3] (ab32004) image](#ab32004b.jpg)
![Western blot - Rab25 antibody [3F12F3] (ab32004) image](#Image2.gif)
![Immunocytochemistry/ Immunofluorescence - Rab25 antibody [3F12F3] (ab32004) image](#Rab25-Primary-antibodies-ab32004-1.jpg)
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Products:Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Overview
Product name
Anti-Rab25 antibody [3F12F3]
See all Rab25 products (3) ...
Description
Mouse monoclonal [3F12F3] to Rab25
Tested applications
IHC-Fr, ELISA, IHC-P, WB, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Ni-NTA purified recombinant human Rab25 expressed in E. Coli strain BL21(DE3).
Positive control
Carcinomas of the skin, bladder, esophagus, breast and ovary.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 0.2% BSA, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Clone number
3F12F3
Isotype
IgG2a
Research areas
Cancer >> Tumor biomarkers >> Oncoproteins
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Applications
Show applications key
Our Abpromise guarantee covers the use of ab32004 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr
IHC-Fr: 1/500 - 1/2000.IHC-Fr: 1/500 - 1/2000.
ELISA
ELISA: 1/10000ELISA: 1/10000
IHC-P: 1/500 - 1/2000.
WB: 1/500 - 1/2000.Detects a band of approximately 28 kDa (predicted molecular weight: 24 kDa).
ICC/IF: Use at an assay dependent dilution. (PubMed: 20717956)
Target
Function
Involved in the regulation of cell survival. Promotes invasive migration of cells in which it functions to localize and maintain integrin alpha-V/beta-1 at the tips of extending pseudopodia. Increases the rates of ovarian and breast cancers progression and aggressiveness by modulating cellular processes such as proliferation, survival and migration of epithelial tumor cells. May selectively regulate the apical recycling pathway. Together with MYO5B regulates transcytosis.
Tissue specificity
Expressed in ovarian epithelium (NOE) and breast tissue. Expressed in ovarian cancer; expression is increased relative to NOE cells. Expression in ovarian cancer is stage dependent, with stage III and stage IV showing higher levels than early stage cancers. Expressed in breast cancer; expression is increased relative to normal breast tissue.
Sequence similarities
Belongs to the small GTPase superfamily. Rab family.
Cellular localization
Cell membrane. Cell projection > pseudopodium membrane. Cytoplasmic vesicle. Colocalizes with integrin alpha-V/beta-1 in vesicles at the pseudopodial tips.
Target information above from: UniProt accessionP57735
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- RP11-336K24.10-001 antibody
- CATX 8 antibody
- CATX-8 antibody
see all
Database links
- Entrez Gene: 57111 Human
- SwissProt: P57735 Human
- SwissProt: Q5VYA2 Human
- Unigene: 632469 Human
Anti-Rab25 antibody [3F12F3] images:
Immunohistochemistry (Paraffin-embedded sections) - Rab25 antibody [3F12F3] (ab32004)
![Immunohistochemistry (Paraffin-embedded sections) - Rab25 antibody [3F12F3] (ab32004)](/ps/datasheet/Images/32/ab32004/ab32004b.jpg)
Immunohistochemisty analysis of parafin embedded carcinoma tissues, using ab32004. The staining shows the cytoplasmic location of Rab25. A DAB staining procedure was used to stain tissue sections.
Western blot - Rab25 antibody [3F12F3] (ab32004)
![Western blot - Rab25 antibody [3F12F3] (ab32004)](/ps/datasheet/Images/32/ab32004/Image2.gif)
All lanes : Anti-Rab25 antibody [3F12F3] (ab32004) at 1/500 dilution
Lane 1 : Human truncated Rab25 recombinant protein
Lane 2 : Ovary Carcinoma tissue lysate
Lane 3 : Stomach Carcinoma tissue lysate
Lane 4 : Breast Carcinoma tissue lysate
Predicted band size : 24 kDa
Observed band size : 28 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - Rab25 antibody [3F12F3] (ab32004)
![Immunocytochemistry/ Immunofluorescence - Rab25 antibody [3F12F3] (ab32004)](/ps/datasheet/images/32/ab32004/Rab25-Primary-antibodies-ab32004-1.jpg)
ICC/IF image of ab32004 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab32004, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab97899 Dylight 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Rab25 antibody [3F12F3] (ab32004)
This product has been referenced in:
- Oehlke Oet al. Rab11b and its effector Rip11 regulate the acidosis-induced traffic of V-ATPase in salivary ducts. J Cell Physiol 226:638-51 (2011). WB, ICC/IF; Human.Read more (PubMed: 20717956) »
See 1 publication for this product
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"