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Immunohistochemistry (Paraffin-embedded sections) - Rab25 antibody [3F12F3] (ab32004)
![Immunohistochemistry (Paraffin-embedded sections) - Rab25 antibody [3F12F3] (ab32004)](/ps/datasheet/images/ab32004b.jpg)
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Western blot - Rab25 antibody [3F12F3] (ab32004)
![Western blot - Rab25 antibody [3F12F3] (ab32004)](/ps/datasheet/images/Image2.gif)
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Product Name
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Rab25 antibody [3F12F3]
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See all Rab25 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [3F12F3] to Rab25
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Immunogen
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Ni-NTA purified recombinant human Rab25 expressed in E. Coli strain BL21(DE3).
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ELISA, IHC-Fr, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/10000.
IHC-Fr: 1/500 - 1/2000.
IHC-P: 1/500 - 1/2000.
WB: 1/500 - 1/2000. Detects a band of approximately 28 kDa (predicted molecular weight: 24 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Carcinomas of the skin, bladder, esophagus, breast and ovary.
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Cellular localization
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Cell Membrane lipid-anchor; cytoplasmic side
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Research areas
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Cancer >> Tumor biomarkers >> Oncoproteins
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
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Relevance
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Rab 25 is a plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. Rab proteins play an important role for either in endocytosis or in biosynthetic protein transport. The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the SEC4 protein, which is 40% similar to Rab proteins, is associated with secretory vesicles. Rab proteins are located on the cytoplasmic face of organelles and vesicles and are involved in intracellular membrane fusion reactions. Rab25 was cloned from a gastric parietal cell cDNA library and is expressed in epithelial tissues such as the gastrointestinal mucosae, kidney, and lung.
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Rab25 antibody [3F12F3] (ab32004) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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3F12F3
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Isotype
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IgG2a
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Purity
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Protein A purified
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Storage buffer
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Preservative: 0.09% Sodium Azide
Constituents: 0.2% BSA, PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Rab25 antibody [3F12F3] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Rab25 antibody [3F12F3] images, references, products related to ab32004 and other tools.
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Rab25 antibody [3F12F3] images:
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 Immunohistochemistry (Paraffin-embedded sections) - Rab25 antibody [3F12F3] (ab32004)
Immunohistochemisty analysis of parafin embedded carcinoma tissues, using ab32004. The staining shows the cytoplasmic location of Rab25.
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Western blot - Rab25 antibody [3F12F3] (ab32004)
All lanes : Rab25 antibody [3F12F3] (ab32004) at 1/500 dilution
Lane 1 : Human truncated Rab25 recombinant protein
Lane 2 : Ovary Carcinoma tissue lysate
Lane 3 : Stomach Carcinoma tissue lysate
Lane 4 : Breast Carcinoma tissue lysate
Predicted band size : 24 kDa
Observed band size : 28 kDa (why is the actual band size different from the predicted?)
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Search PubMed (MEDLINE) for references to Rab25
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Rab25 antibody [3F12F3] - more information
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Customer reviews (feedback) regarding Rab25 antibody [3F12F3] |
Customer FAQs regarding Rab25 antibody [3F12F3] |
Protocols for Rab25 antibody [3F12F3] |
Price and availability of products related to Rab25 antibody [3F12F3] |
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Rab25 antibody [3F12F3] - related products:
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Rab25 antibody [3F12F3] - other tools:
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Contact Abcam with a Technical Enquiry about Rab25 antibody [3F12F3] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
![Immunohistochemistry (Paraffin-embedded sections) - Rab25 antibody [3F12F3] (ab32004)](/index.html?datasheet=32004#ab32004b.jpg)
![Western blot - Rab25 antibody [3F12F3] (ab32004)](/index.html?datasheet=32004#Image2.gif)
