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Immunocytochemistry - Rab4 antibody - Early Endosome Marker (ab13252)
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Western blot - Rab4 antibody - Early Endosome Marker (ab13252)
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Product Name
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Rab4 antibody - Early Endosome Marker
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See all Rab4 - Early Endosome Marker antibodies (6)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Rab4 - Early Endosome Marker
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Immunogen
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Recombinant fusion protein (Human) expressed in E.coli.
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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This antibody is very specific for Rab4. To our knowledge this antibody does not cross-react with other Rab proteins. However, as there are dozens
of different proteins in the Rab family, not every single one has been tested for cross-reactivity with this antibody.
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Tested applications
(see key)
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ICC/IF, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC: 1/200 - 1/500.
IP: 1/100.
WB: 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 25.6 kDa).
ICC/IF: Use at an assay dependent dilution (from PubMed:17547697).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Mouse or Rat Brain Tissue.
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Cellular localization
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Peripheral membrane protein, generally associated with membranes. Cytoplasmic when phosphorylated by CDC2.
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Research areas
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Cancer >> Signal transduction >> G protein signaling >> Small G proteins >> Other
Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Other
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Endosome
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Relevance
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Rab4 is a small GTPase that regulates recycling of proteins, such as GPCRs, from the early endosomes to the cell surface. Overexpression of Rab4 causes a redistribution of receptors on plasma membrane versus endocytic compartments. Rab4 is also involved in the translocation of glucose transporter (Glu4) in adipocytes in response to insulin. Association of Rab4 with transferrin receptor containing early endosomes is mediated through the geranylgeranyl groups at its carboxyl terminus. Rab4 can be used as a marker for early endosomes (see Daro et al. 1996 and Zuk et al. 1999 for more information). Membrane association is also cell cycle dependent, as phosphorylation at its C-terminal cdc2 kinase consensus sequence in mitotic cells leads to dissociation of Rab4 into the cytosol.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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Preservative: None
Constituents: Whole Serum
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Rab4 antibody - Early Endosome Marker is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Rab4 antibody - Early Endosome Marker images, references, products related to ab13252 and other tools.
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Rab4 antibody - Early Endosome Marker images:
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 Immunocytochemistry - Rab4 antibody - Early Endosome Marker (ab13252)
ab13252 Rabbit polyclonal to Rab4 was used in fixed primary cultures of rat hippocampal neurons (embryonic day 18) at a dilution of 1/200. A secondary goat anti-rabbit antibody was used for detection (Alexa Fluor 488; 1/200; green). Confocal microscopy revealed typically punctuate immunofluorescence expected of Rab4 in these neurons. The red colour labelling is that of monoclonal mouse anti-b-tubulin-II. Magnification 180x. Randal Moldrich, CNRS UMR7637, ESPCI, France
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Western blot - Rab4 antibody - Early Endosome Marker (ab13252)
Rab4 antibody - Early Endosome Marker (ab13252) at 1/1000 dilution + Lysates prepared from rat brain tissue
Predicted band size : 25.6 kDa
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References for Rab4 antibody - Early Endosome Marker (ab13252)
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This product has been used in: (two most recent references)
Hall SL et al. The organelle proteome of the DT40 lymphocyte cell line. Mol Cell Proteomics 8:1295-305 (2009). PubMed: 19181659
McCaffrey G et al. High-resolution fractionation of signaling endosomes containing different receptors. Traffic 10:938-50 (2009). WB; Human. PubMed: 19416476
See all 6 publication references for this product.
If you publish research using ab13252 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Rab4
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Rab4 antibody - Early Endosome Marker - more information
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Rab4 antibody - Early Endosome Marker - related products:
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Rab4 antibody - Early Endosome Marker - other tools:
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Contact Abcam with a Technical Enquiry about Rab4 antibody - Early Endosome Marker |
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