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Products:Neuroscience >> Neurotransmission >> Secretory Vesicles >> Rabs
Overview
Product name
Anti-Rab5 antibody - Early Endosome Marker
See all Rab5 products (10) ...
Description
Rabbit polyclonal to Rab5 - Early Endosome Marker
Tested applications
WB, ICC/IF, IHC-P, ICCmore details
Cross reactivity
Reacts with
Mouse, Rat, Hamster, Cow, Human, Monkey
Predicted to work with
Dog
Immunogen
Synthetic peptide: PKNEPQNPGANSARGR conjugated to KLH, corresponding to amino acids 182-197 of Human Rab5 (Rab5A)
PKNEPQNPGA NSARGR
Positive control
Mouse or Rat Brain Tissue.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 50% Glycerol, 1X PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Endosome
Neuroscience >> Neurotransmission >> Secretory Vesicles >> Rabs
Applications
Show applications key
Our Abpromise guarantee covers the use of ab13253 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF
- 1 Image
IHC-P
Application notes
ICC/IF: Use at an assay dependent dilution (PMID 19348413).
IHC-P: 1/100 (use Bouin's fixative to fix tissue).
WB: 1/2000. Detects a band of approximately 26 kDa (predicted molecular weight: 25.8 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Required for the fusion of plasma membranes and early endosomes.
Sequence similarities
Belongs to the small GTPase superfamily. Rab family.
Cellular localization
Cell membrane. Early endosome membrane. Melanosome. Enriched in stage I melanosomes.
Target information above from: UniProt accessionP20339
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- RAB 5 antibody
- RAB 5A antibody
- RAB5A antibody
see all
Database links
- Entrez Gene: 539764 Cow
- Entrez Gene: 404008 Dog
- Entrez Gene: 5868 Human
- Entrez Gene: 271457 Mouse
- Entrez Gene: 64633 Rat
- Omim: 179512 Human
- SwissProt: Q0IIG7 Cow
- SwissProt: P18066 Dog
see all
Anti-Rab5 antibody - Early Endosome Marker images:
Immunocytochemistry - Rab5 antibody - Early Endosome Marker (ab13253)
Confocal microscopy of fixed primary cultures of rat hippocampal neurons (embryonic day 18) showing immunocytochemical labelling of polyclonal rabbit anti-Rab5 (Abcam ab13253, 1/200; Alexa Fluor 488; 1/200; green) and monoclonal mouse anti-tubulin-II (Alexa Fluor 568 1/200; red). Magnification 63x; insert 180x.
Randal Moldrich, CNRS UMR7637, ESPCI, France
Immunocytochemistry - Rab5 antibody - Early Endosome Marker (ab13253)
ab13253 Rabbit polyclonal to Rab5 was used in in fixed HEK cells that had been transfected with Rab5-GFP Fluorescence microscopy of fixed Rab5-transfected HEK cells showing (left to right) immunocytochemical labelling of rabbit polyclonal Rab5 (ab13253, 1/500; Cy3; red), and Rab5-GFP (green) with their overlay (far right). Scale bar = 7µm. Showing good co-localisation.
Randal Moldrich, CNRS UMR7637, ESPCI, France
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rab5 antibody - Early Endosome Marker (ab13253)
ab13253 at 1/100 dilution staining Rab5 in mouse back skin tissue section by Immunohistochemistry (Bouin's fixative fixed paraffin-embedded tissue section). Antigen retrieval was done by microwave in citrate buffer. A Fluorophore-conjugated goat anti-rabbit secondary was used at 1/50 dilution.
References for Anti-Rab5 antibody - Early Endosome Marker (ab13253)
This product has been referenced in:
- Brodin Pet al. High content phenotypic cell-based visual screen identifies Mycobacterium tuberculosis acyltrehalose-containing glycolipids involved in phagosome remodeling. PLoS Pathog 6: (2010). ICC/IF; Mouse.Read more (PubMed: 20844580) »
- Källquist Let al. Neutrophil elastase and proteinase 3 trafficking routes in myelomonocytic cells. Exp Cell Res 316:3182-96 (2010). ICC/IF; Human.Read more (PubMed: 20828556) »
See all 7 publications for this product
Publishing research using ab13253? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"