Products:Tags & Cell Markers >> Subcellular Markers >> Organelles >> Endosome
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ab50533 has been referenced in 11 publications.
Publishing research using ab50533? Please let us know so that we can cite the reference in this datasheet
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ICC/IF image of ab50533 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab50533, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-RAB7 antibody [Rab7-117] (ab50533) at 1/1000 dilution + Lysate prepared from mouse neuroblastoma cells at 10 µg
Secondary
HRP-conjugated goat monoclonal to mouse IgG at 1/2000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 23 kDa
Observed band size : 23 kDa
Exposure time : 20 seconds
This image is a courtesy of Anonymous Abreview
Anti-RAB7 antibody [Rab7-117] (ab50533) at 1/500 dilution (for 3 hours at 20°C) + Human brain whole tissue lysate at 15 µg
Secondary
An HRP-conjugated Goat anti-mouse IgG polyclonal at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 23 kDa
Observed band size : 24 kDa (why is the actual band size different from the predicted?)
Exposure time : 1 minute
Blocking Step: 5% Milk for 1 hour at 20°C
This image is courtesy of an anonymous Abreview
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