RAB7 antibody [Rab7-117] (ab50533)

Product Name 

RAB7 antibody [Rab7-117]

Product type 

Primary antibodies

Description 

Mouse monoclonal [Rab7-117] to RAB7

Immunogen 

Synthetic peptide: EQAFQTIARNALKQE, corresponding to amino acids 163-177 of Human RAB7
BLAST 'EQAFQTIARNALKQE' with or

Reacts with 

Hu, Ms, Rat, Chk, Cow, Mk

Predicted to react 
(species key)

Dog, Rb, Xl, Zfsh
(Due to sequence homology)

Tested applications 
(see key)

ELISA, ICC/IF, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependent dilution.
ICC: Use at an assay dependent dilution.
ICC/IF: Use at a concentration of 1 µg/ml.
IP: Use at an assay dependent dilution.
WB: Use at a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 23 kDa.
Array: Use at an assay dependent dilution.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

HeLa total cell extract.

Cellular localization 

Endosome; late endosome. Lysosome. Cytoplasmic vesicle; phagosome. Melanosome.

Research areas 

Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Lysosome
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Endosome

Relevance 

Members of the RAB family of RAS related GTP binding proteins are important regulators of vesicular transport and are located in specific intracellular compartments. RAB7 has been localized to late endosomes and shown to be important in the late endocytic pathway. In addition, it has been shown to have a fundamental role in the cellular vacuolation induced by the cytotoxin VacA of Helicobacter pylori.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

Rab7-117

Myeloma 

NS1

Isotype 

IgG2b

Storage buffer 

Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Purified immunoglobulin.

Form 

Liquid

Concentration 

Batch dependent within range: 2.40 - 2.40 mg/ml

Concentration of lot no.     is  

Concentration not available for this lot.

Find concentration of your lot :

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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RAB7 antibody [Rab7-117] images:

References for RAB7 antibody [Rab7-117] (ab50533)

  This product has been used in: (two most recent references)

Ha SD et al. Cathepsin B-mediated autophagy flux facilitates the anthrax toxin receptor 2-mediated delivery of anthrax lethal factor into the cytoplasm. J Biol Chem 285:2120-9 (2010). WB; Mouse. PubMed: 19858192

Maryon EB et al. Human copper transporter 1 lacking O-linked glycosylation is proteolytically cleaved in a Rab9-positive endosomal compartment. J Biol Chem 284:28104-14 (2009). ICC/IF; Human. PubMed: 19684018

See all 4 publication references for this product.


If you publish research using ab50533 please let us know so that we can cite the reference on this datasheet.

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