Anti-RAB14 antibody (ab28639)

Overview

• Product nameAnti-RAB14 antibodySee all RAB14 primary antibodies ...
• Description
  Rabbit polyclonal to RAB14
• Tested applicationsICC/IF, ELISA, IHC-P, WB more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat, Cow, Dog, Pig, Zebrafish
• Immunogen

  Synthetic peptide derived from a region between amino acids 165-214 of human RAB14. (Note: the exact amino acid sequence is proprietary).

• Positive controlRaji cell lysate, Human kidney tissue

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: 2% Sucrose, PBS
• Concentration information loading...
• PurityIgG fraction
• Purification notesPurified by peptide affinity chromatography method.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Protein Trafficking
  • Golgi Proteins

  • Signal Transduction
  • Protein Trafficking
  • Organelle Proteins

Applications

Target

• FunctionMay be involved in vesicular trafficking and neurotransmitter release.
• Sequence similaritiesBelongs to the small GTPase superfamily. Rab family.
• Cellular localizationCell membrane.

Target information above from: UniProt accession P61106 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 51552 Human
  • Entrez Gene: 68365 Mouse
  • Entrez Gene: 595112 Pig
  • Entrez Gene: 94197 Rat
  • Entrez Gene: 373104 Zebrafish
  • Omim: 612673 Human
  • SwissProt: P61106 Human
  • SwissProt: Q50HW9 Mouse

  • SwissProt: Q50HX0 Mouse
  • SwissProt: Q50HX1 Mouse
  • SwissProt: Q50HX2 Mouse
  • SwissProt: Q50HX3 Mouse
  • SwissProt: Q50HX4 Mouse
  • SwissProt: Q91V41 Mouse
  • SwissProt: P61107 Rat
  • SwissProt: Q7ZVX4 Zebrafish
  • Unigene: 371563 Human
  • Unigene: 713585 Human
  • Unigene: 198264 Mouse
  • Unigene: 6008 Rat

  see all

• Alternative names
  bA165P4.3 antibodyF protein binding protein 1 antibodyF protein binding protein 1 antibody

  FBP antibodyGTPase Rab14 antibodyRAB 14 antibodyRAB14 antibodyRAB14 member RAS oncogene family antibodyRAB14_HUMAN antibodyRas related protein Rab 14 antibodyRas-related protein Rab-14 antibodyRP11 165P4.4 antibodySmall GTP binding protein RAB14 antibody

  see all

Anti-RAB14 antibody images

• 

  Western blot - RAB14 antibody (ab28639)
  Anti-RAB14 antibody (ab28639) at 0.5 µg/ml + Raji cell lysate 10ug.
  
  Secondary
  HRP conjugated anti-Rabbit IgG
  
  Predicted band size : 24 kDa
  Observed band size : 22 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunohistochemistry (Paraffin-embedded sections) - RAB14 antibody (ab28639)
  Human kidney tissue stained with ab28639 at X400 magnigication. The arrows point to epithelial cells of the renal tubule [positive label].
• 

  Immunocytochemistry/ Immunofluorescence - Anti-RAB14 antibody (ab28639)
  ICC/IF image of ab28639 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28639, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.