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ab3612 has been referenced in 7 publications.
Publishing research using ab3612? Please let us know so that we can cite the reference in this datasheet
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ab3612 staining HEK 293 cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.012% saponin prior to blocking in 1% BSA for 30 minutes at 37°C. The primary antibody was diluted 1/200 and incubated with the sample for 2 hours at 37°C. A biotinylated goat anti-rabbit antibody, diluted 1/200, was used as the secondary.
This image depicts ab3612 recognizing endogenous Rab11 (red). Rab11 was used as a recycling endosome marker.
This image is courtesy of an anonymous Abreview
ab3612 staining Rab11 in canine kidney cells by Immunocytochemistry/ Immunofluorescence.Cells were fixed in paraformaldehyde, permeabilized using 0.5% saponin, then incubated with ab3612 at a 1/200 dilution for 1.5 hours at 20°C. The secondary used was an Alexa-Fluor 568 conjugated donkey anti-rabbit polyclonal, used at a 1/350 dilution.
Image courtesy of an anonymous Abreview.
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