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Western blot - Rab25 antibody (ab45855)
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Western blot - Rab25 antibody (ab45855)
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Immunocytochemistry/ Immunofluorescence - Rab25 antibody (ab45855)
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Product Name
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Rab25 antibody
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See all Rab25 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Rab25
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human Rab25. (Peptide available as ab69566.)
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Reacts with
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Hu, Rat
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Predicted to react
(species key)
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Chmp, Dog, Hrs
(Due to sequence homology)
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 5 µg/ml.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 23 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive signal in the following Human Whole Cell lysates:
MDA-MB-361, MCF7, T47D, HeLa, HepG2, Y79, SW480 and Jurkat
This antibody also gave a positive signal in the following Tissue lysates:
Human Breast Tumour and Rat Thymus
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Cellular localization
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Cell Membrane lipid-anchor; cytoplasmic side
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Research areas
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Cancer >> Tumor biomarkers >> Oncoproteins
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
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Relevance
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Rab 25 is a plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. Rab proteins play an important role for either in endocytosis or in biosynthetic protein transport. The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the SEC4 protein, which is 40% similar to Rab proteins, is associated with secretory vesicles. Rab proteins are located on the cytoplasmic face of organelles and vesicles and are involved in intracellular membrane fusion reactions. Rab25 was cloned from a gastric parietal cell cDNA library and is expressed in epithelial tissues such as the gastrointestinal mucosae, kidney, and lung.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.700 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Rab25 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Rab25 antibody images, references, products related to ab45855 and other tools.
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Rab25 antibody images:
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 Western blot - Rab25 antibody (ab45855)
All lanes : Rab25 antibody (ab45855) at 1 µg/ml
Lane 1 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 3 : T47D (Human ductal breast epithelial tumor cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 23 kDa
Observed band size : 32 kDa (why is the actual band size different from the predicted?)
Additional bands at : 16 kDa,45 kDa,75 kDa. We are unsure as to the identity of these extra bands.
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Western blot - Rab25 antibody (ab45855)
All lanes : Rab25 antibody (ab45855) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
Lane 4 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate (ab3957)
Lane 5 : Rat Thymus Tissue Lysate
Lane 6 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 7 : Breast (Human) Tissue Lysate (ab30091)
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 23 kDa
Observed band size : 36 kDa (why is the actual band size different from the predicted?)
Additional bands at : 17 kDa,50 kDa,70 kDa. We are unsure as to the identity of these extra bands.
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Immunocytochemistry/ Immunofluorescence - Rab25 antibody (ab45855)
ICC/IF image of ab45855 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab45855, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) Hek293 cells at 5µg/ml, and in 4% PFA fixed (10 min) HeLa and MCF7 cells at 5µg/ml.
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Search PubMed (MEDLINE) for references to Rab25
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Rab25 antibody - more information
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Customer reviews (feedback) regarding Rab25 antibody |
Customer FAQs regarding Rab25 antibody |
Protocols for Rab25 antibody |
Price and availability of products related to Rab25 antibody |
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Rab25 antibody - related products:
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Rab25 antibody - other tools:
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Contact Abcam with a Technical Enquiry about Rab25 antibody |
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