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If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab68340? Please let us know so that we can cite the reference in this datasheet
ab68340 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - Rab9 antibody (ab68340)
Anti-Rab9 antibody (ab68340) at 5 µg/ml + immunogen at 0.2 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
Predicted band size : 23 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?)
Note: MW of GST-tag alone is 26 kDa
![Western blot - Rab9 antibody [1E12] (ab68340)](/ps/datasheet/Images/68/ab68340/Wb rab9 2.jpg)
Western blot - Rab9 antibody [1E12] (ab68340)
Anti-Rab9 antibody (ab68340) at 5 µg/ml + HeLa cell lysate at 25 µg/ml
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 23 kDa
Observed band size : 23 kDa
Additional bands at : 33 kDa. We are unsure as to the identity of these extra bands.
![Immunocytochemistry/ Immunofluorescence - Rab9 antibody [1E12] (ab68340)](/ps/datasheet/Images/68/ab68340/IF rab9.jpg)
Immunocytochemistry/ Immunofluorescence - Rab9 antibody [1E12] (ab68340)
Immunofluorescence of monoclonal antibody to Rab9 ab68340 on HeLa cells. Antibody concentration 10 ug/ml.
ELISA - Rab9 antibody (ab68340)
Detection limit for recombinant GST tagged Rab9 is approximately 0.1ng/ml as a capture antibody.
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