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If your product does not perform as described on this datasheet, we will refund or replace your product...
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Western blot - Rab9 antibody (ab68340)
Anti-Rab9 antibody (ab68340) at 5 µg/ml + immunogen at 0.2 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
Predicted band size : 23 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?)
Note: MW of GST-tag alone is 26 kDa
![Western blot - Rab9 antibody [1E12] (ab68340)](/ps/datasheet/Images/68/ab68340/Wb rab9 2.jpg)
Western blot - Rab9 antibody [1E12] (ab68340)
Anti-Rab9 antibody (ab68340) at 5 µg/ml + HeLa cell lysate at 25 µg/ml
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 23 kDa
Observed band size : 23 kDa
Additional bands at : 33 kDa. We are unsure as to the identity of these extra bands.
![Immunocytochemistry/ Immunofluorescence - Rab9 antibody [1E12] (ab68340)](/ps/datasheet/Images/68/ab68340/IF rab9.jpg)
Immunocytochemistry/ Immunofluorescence - Rab9 antibody [1E12] (ab68340)
Immunofluorescence of monoclonal antibody to Rab9 ab68340 on HeLa cells. Antibody concentration 10 ug/ml.
ELISA - Rab9 antibody (ab68340)
Detection limit for recombinant GST tagged Rab9 is approximately 0.1ng/ml as a capture antibody.
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