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Products:Immunology >> Adaptive Immunity >> T Cells >> Cytotoxic Cells
Overview
Product name
Anti-Rab34 antibody
See all Rab34 products (2) ...
Description
Rabbit polyclonal to Rab34
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Cow, Pig
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Rab34.
(Peptide available as ab87820.)
Positive control
This antibody gave a positive signal in the following lysates: Tissue: Mouse brain; Human liver; Rat brain. Whole cell: A431; F9
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Endosome
Immunology >> Adaptive Immunity >> T Cells >> Cytotoxic Cells
Applications
Show applications key
Our Abpromise guarantee covers the use of ab73383 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 1 µg/ml
WB: Use a concentration of 1 µg/mlDetects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
Target
Function
Protein transport. Involved in the redistribution of lysosomes to the peri-Golgi region.
Sequence similarities
Belongs to the small GTPase superfamily. Rab family.
Cellular localization
Cytoplasm. Golgi apparatus. Associates with the Golgi complex.
Target information above from: UniProt accessionQ9BZG1
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Rab-39 antibody
- RAB34 antibody
- RAB34, member RAS oncogene family antibody
see all
Database links
- Entrez Gene: 83871 Human
- Entrez Gene: 19376 Mouse
- Entrez Gene: 360571 Rat
- SwissProt: Q9BZG1 Human
- SwissProt: Q64008 Mouse
- SwissProt: Q5U1Y1 Rat
- Unigene: 181291 Human
- Unigene: 301853 Human
see all
Anti-Rab34 antibody images:
Western blot - Rab34 antibody (ab73383)
All lanes : Anti-Rab34 antibody (ab73383) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : Brain (Rat) Tissue Lysate
Lane 5 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 29 kDa
Observed band size : 29 kDa
Additional bands at : 18 kDa,58 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 2 minutes
Immunocytochemistry/ Immunofluorescence - Rab34 antibody (ab73383)
ICC/IF image of ab73383 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab73383, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% Methanol fixed (5 min) HeLa cells at 1µg/ml.
References for Anti-Rab34 antibody (ab73383)
ab73383 has not yet been referenced specifically in any publications.
Publishing research using ab73383? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"