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I used ab77993 to stain HEP2 and African green moneky cells by ICC/IF and didn't see any staining. |
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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
ab77993 at 10µg/ml staining RAB7 in skeletal muscle tissue by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.
All lanes : Anti-RAB7 antibody (ab77993) at 1 µg/ml
Lane 1 :
Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 23 kDa
Observed band size : 23 kDa
Additional bands at : 34 kDa,49 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 1 minute
1
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