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Products:Tags & Cell Markers >> Subcellular Markers >> Organelles >> Endosome
Overview
Product name
Anti-RAB7 antibody
See all RAB7 products (5) ...
Description
Rabbit polyclonal to RAB7
Tested applications
ICC/IF, WB, IHC-Pmore details
Cross reactivity
Reacts with
Rat, Chicken, Cow, Dog, Human, Xenopus laevis, Chimpanzee, Monkey, Zebrafish
Immunogen
Synthetic peptide, corresponding to a portion of the amino acids 90-140 of Human RAB7A
Positive control
Skeletal muscle tissue.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 0.2% Gelatin, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Lysosome
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Endosome
Applications
Show applications key
Our Abpromise guarantee covers the use of ab77993 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent dilution. (PubMed: 21191022)
WB: Use a concentration of 0.5 - 1 µg/ml.Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
IHC-P: Use a concentration of 10 µg/ml
Target
Relevance
Members of the RAB family of RAS related GTP binding proteins are important regulators of vesicular transport and are located in specific intracellular compartments. RAB7 has been localized to late endosomes and shown to be important in the late endocytic pathway. In addition, it has been shown to have a fundamental role in the cellular vacuolation induced by the cytotoxin VacA of Helicobacter pylori.
Cellular localization
Endosome; late endosome. Lysosome. Cytoplasmic vesicle; phagosome. Melanosome.
Alternative names
- MGC16212 antibody
- CMT2B antibody
- Member RAS oncogene family antibody
see all
Database links
- Entrez Gene: 416016 Chicken
- Entrez Gene: 281439 Cow
- Entrez Gene: 404007 Dog
- Entrez Gene: 7879 Human
- Entrez Gene: 29448 Rat
- Entrez Gene: 446841 Xenopus laevis
- Entrez Gene: 393902 Zebrafish
- Omim: 602298 Human
see all
Anti-RAB7 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RAB7 antibody (ab77993)
ab77993 at 10µg/ml staining RAB7 in skeletal muscle tissue by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.
Western blot - RAB7 antibody (ab77993)
All lanes : Anti-RAB7 antibody (ab77993) at 1 µg/ml
Lane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)
Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 23 kDa
Observed band size : 23 kDa
Additional bands at : 34 kDa,49 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 1 minute
References for Anti-RAB7 antibody (ab77993)
This product has been referenced in:
- Wolf Met al. Rhesus rotavirus entry into a polarized epithelium is endocytosis dependent and involves sequential VP4 conformational changes. J Virol 85:2492-503 (2011). ICC/IF; Dog.Read more (PubMed: 21191022) »
See 1 publication for this product
Publishing research using ab77993? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"