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Products:Neuroscience >> Neurotransmission >> Secretory Vesicles >> Rabs
Overview
Product name
Anti-RAB23 antibody
See all RAB23 products (3) ...
Description
Goat polyclonal to RAB23
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Mouse
Predicted to work with
Human
Immunogen
Synthetic peptide: C-PNKQRTKKNRNP, corresponding to C terminal amino acids 219-230 of human RAB23 (NP_057361.3; NP_899050.1).
C-PNKQRTKK NRNP
Positive control
Mouse eye lysates.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab82165 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Primary antibody notes
Both reported variants (NP_057361.3; NP_899050.1) represent identical protein.
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Signaling Pathways >> Hedgehog >> Surface Molecules
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Signal Transduction >> Second Messenger >> Nucleotide Messenger >> GTP
Neuroscience >> Neurotransmission >> Secretory Vesicles >> Rabs
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Western blot - RAB23 antibody (ab82165)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab82165 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ELISA
Application notes
Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1:64,000.
WB: Use at a concentration of 1 - 3 µg/ml. Predicted molecular weight: 27 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Involvement in disease
Defects in RAB23 are the cause of acrocephalopolysyndactyly type 2 (ACPS2) [MIM:201000]. A syndrome characterized by craniosynostosis, polysyndactyly, obesity, and cardiac defects.
Sequence similarities
Belongs to the small GTPase superfamily. Rab family.
Cellular localization
Cell membrane.
Target information above from: UniProt accessionQ9ULC3
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DKFZp781H0695 antibody
- HSPC137 antibody
- MGC8900 antibody
see all
Database links
- Entrez Gene: 51715 Human
- Entrez Gene: 19335 Mouse
- Omim: 606144 Human
- SwissProt: Q9ULC3 Human
- SwissProt: P35288 Mouse
- Unigene: 555016 Human
- Unigene: 86744 Mouse
Anti-RAB23 antibody images:
Western blot - RAB23 antibody (ab82165)
Anti-RAB23 antibody (ab82165) at 0.1 µg/ml + Mouse Eye lysate at 35 µg
Predicted band size : 27 kDa
Observed band size : 30 kDa (why is the actual band size different from the predicted?)
The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources.
References for Anti-RAB23 antibody (ab82165)
ab82165 has not yet been referenced specifically in any publications.
Publishing research using ab82165? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"