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Western blot - R2D2 antibody (ab27650)
(enlarge)
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Product Name
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R2D2 antibody
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See all R2D2 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to R2D2
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Fruit fly (Drosophila melanogaster) R2D2.
(Peptide available as ab17670.)
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Reacts with
(species key)
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Dm
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Specificity
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The antibody specifically recognises the immunising peptide in a blocking western blot. All four observed bands are blocked, showing that they are specific. There is also low background and high titre in ELISA. Please let us know if you can explain these bands.
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Tested applications
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 35 kDa). Can be blocked with R2D2 peptide (ab17670).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> RNAi >> RISC
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Relevance
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The RNA interference (RNAi) pathway is initiated by processing long double-stranded RNA into small interfering RNA (siRNA). The siRNA-generating enzyme consists of DCR-2 and R2D2. R2D2 is homologous to the Caenorhabditis elegans RNAi protein RDE-4.
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Database links
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The links below go to external sites and will open in a new browser window Entrez Gene | | 34066 (Fruit fly (Drosophila melanogaster))
| SwissProt | | Q9VLW8 (Fruit fly (Drosophila melanogaster))
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| Unigene | | 10740 (Fruit fly (Drosophila melanogaster))
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.350 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this R2D2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for R2D2 antibody images, references, products related to ab27650 and other tools.
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R2D2 antibody images:
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 Western blot - R2D2 antibody (ab27650)
All lanes : R2D2 antibody (ab27650) at 1 µg/ml
Lane 1 : Drosophila lysate
Lane 2 : Drosophila lysate with R2D2 peptide (ab17670) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Predicted band size : 35 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)
Additional bands at : 39 kDa (possible cross reactivity),55 kDa (possible cross reactivity),85 kDa (possible cross reactivity).
ab27650 specifically recognises a band at 37kDa corresponding to R2D2, which is blocked using the immunising peptide (ab17670).
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Search PubMed (MEDLINE) for references to R2D2
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R2D2 antibody - more information
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Customer reviews (feedback) regarding R2D2 antibody |
Customer FAQs regarding R2D2 antibody |
Protocols for R2D2 antibody |
Price and availability of products related to R2D2 antibody |
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R2D2 antibody - related products:
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R2D2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about R2D2 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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