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Anti-R2D2 antibody (ab27650)

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Overview

Product name

Anti-R2D2 antibody
See all R2D2 products (2) ...

Description

Rabbit polyclonal to R2D2

Specificity

The antibody specifically recognises the immunising peptide in a blocking western blot. All four observed bands are blocked, showing that they are specific. There is also low background and high titre in ELISA. Please let us know if you can explain these bands.

Tested applications

WBmore details

Cross reactivity

Reacts with

Fruit fly (Drosophila melanogaster)

Immunogen

Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Fruit fly (Drosophila melanogaster) R2D2.

Read Abcam's proprietary immunogen policy

(Peptide available as ab17670.)

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> RNAi >> RISC

  • Western blot - R2D2 antibody (ab27650)Western blot - R2D2 antibody (ab27650) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab27650 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

The RNA interference (RNAi) pathway is initiated by processing long double-stranded RNA into small interfering RNA (siRNA). The siRNA-generating enzyme consists of DCR-2 and R2D2. R2D2 is homologous to the Caenorhabditis elegans RNAi protein RDE-4.

Cellular localization

Nuclear

Alternative names

  • CG7138 antibody
  • CG7138 PA antibody
  • LD06392p antibody

Database links

Anti-R2D2 antibody images:

  Western blot - R2D2 antibody (ab27650)

Western blot - R2D2 antibody (ab27650)

All lanes : Anti-R2D2 antibody (ab27650) at 1 µg/ml

Lane 1 : Drosophila lysate
Lane 2 : Drosophila lysate with R2D2 peptide (ab17670) at 1 µg/ml

Lysates/proteins at 20 µg per lane.


Predicted band size : 35 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)
Additional bands at : 39 kDa (possible cross reactivity),55 kDa (possible cross reactivity),85 kDa (possible cross reactivity).

ab27650 specifically recognises a band at 37kDa corresponding to R2D2, which is blocked using the immunising peptide (ab17670).

References for Anti-R2D2 antibody (ab27650)

ab27650 has not yet been referenced specifically in any publications.

Publishing research using ab27650? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"