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Western blot - R2D2 peptide (ab17670)
(enlarge)
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Product Name
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R2D2 peptide
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Product type
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Proteins and Peptides
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Peptide
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Synthetic peptide derived from within residues 100 - 200 of Human R2D2.
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Tested applications
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BL
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Application notes
(see key)
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Recommended dilutions
BL: Use at a concentration of 1 µg/ml.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Research areas
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Chromatin and Nuclear Signaling >> RNAi >> RISC
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Relevance
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The RNA interference (RNAi) pathway is initiated by processing long double-stranded RNA into small interfering RNA (siRNA). The siRNA-generating enzyme consists of DCR-2 and R2D2. R2D2 is homologous to the Caenorhabditis elegans RNAi protein RDE-4.
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Storage buffer
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Preservative: None
Constituents: 0.001% Tween 20, HEPES, 2mM EDTA, 150mM Sodium chloride
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this R2D2 peptide is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for R2D2 peptide images, references, products related to ab17670 and other tools.
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R2D2 peptide images:
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 Western blot - R2D2 peptide (ab17670)
All lanes : R2D2 antibody (ab27650) at 1 µg/ml
Lane 1 : Drosophila lysate
Lane 2 : Drosophila lysate with R2D2 peptide (ab17670) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Observed band size : 37 kDa (why is the actual band size different from the predicted?)
Additional bands at : 39 kDa (possible cross reactivity),55 kDa (possible cross reactivity),85 kDa (possible cross reactivity).
ab27650 specifically recognises a band at 37kDa corresponding to R2D2, which is blocked using the immunising peptide (ab17670).
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Search PubMed (MEDLINE) for references to R2D2
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R2D2 peptide - more information
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Customer reviews (feedback) regarding R2D2 peptide |
Customer FAQs regarding R2D2 peptide |
Protocols for R2D2 peptide |
Price and availability of products related to R2D2 peptide |
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R2D2 peptide - related products:
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R2D2 peptide - other tools:
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Contact Abcam with a Technical Enquiry about R2D2 peptide |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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