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Overview
Product name
Anti-R3HDM2 antibody
See all R3HDM2 products (2) ...
Description
Rabbit polyclonal to R3HDM2
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Cow, Dog
Immunogen
Synthetic peptide corresponding to a region within internal sequence amino acids 827-876(QSTYTVHQGQ SGLKHGNRGK RQALKSASTD LGTADVVLGR VLEVTDLPEG) of Human R3HDM2 (NP_055740).
Positive control
293T cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
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Western blot - R3HDM2 antibody (ab83335)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab83335 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ELISA
Application notes
ELISA: Use at an assay dependent dilution. ELISA titre using peptide based assay 1/62500.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 60 and 65 kDa (predicted molecular weight: 105 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
The R3H motif is characterised by the presence of an invariant arginine residue and a highly conserved histidine residue, which are separated by three residues. The function of the domain is predicted to be binding ssDNA. There are two named isoforms of R3HDM2.
Cellular localization
Nuclear
Alternative names
- KIAA1002 antibody
- R3H domain containing protein 2 antibody
- R3H domain protein antibody
Database links
- Entrez Gene: 22864 Human
- Entrez Gene: 71750 Mouse
- Entrez Gene: 362894 Rat
- SwissProt: Q9Y2K5 Human
- SwissProt: Q80TM6 Mouse
- Unigene: 443673 Human
Anti-R3HDM2 antibody images:
Western blot - R3HDM2 antibody (ab83335)
Anti-R3HDM2 antibody (ab83335) at 1 µg/ml + 293T cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 105 kDa
Observed band size : 60,65 kDa (why is the actual band size different from the predicted?)
Gel concentration 12%
References for Anti-R3HDM2 antibody (ab83335)
ab83335 has not yet been referenced specifically in any publications.
Publishing research using ab83335? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"