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Anti-Rab11A antibody [47] (ab78337)

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Overview

Product name

Anti-Rab11A antibody [47]
See all Rab11A products (5) ...

Description

Mouse monoclonal [47] to Rab11A

Tested applications

Flow Cyt, ICC/IF, WBmore details

Cross reactivity

Reacts with

Mouse, Rat, Chicken, Dog, Human, Monkey

Immunogen

Recombinant fragment, corresponding to amino acids 86-207 of Human Rab11A

Positive control

HEK293 cell lysate. HeLa cell lysate. Rat adrenal gland.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.09% Sodium Azide
Constituents: 30% Glycerol, PBS

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Monoclonal

Clone number

47

Isotype

IgG2a

Research areas

Cancer >> Signal transduction >> G protein signaling >> Small G proteins >> Ras family
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family

  • Immunocytochemistry/ Immunofluorescence - Rab11A antibody [47] (ab78337)Immunocytochemistry/ Immunofluorescence - Rab11A antibody [47] (ab78337) image (enlarge)

  • Flow Cytometry-Rab11A antibody [47](ab78337)Flow Cytometry-Rab11A antibody [47](ab78337) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab78337 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    Flow Cyt

      See more...Read more →

    Flow Cyt: Use 1µg for 106 cells.

  • ShowHide3 Images

    ICC/IF

    Four stars (3 Abreviews) ICC/IF: Use at an assay ...Read more →

    ICC/IF: Use at an assay dependent dilution. (This antibody has been reported by an independent laboratory to produce positive staining for Rab11A in ICC/IF using rat adrenal gland (Steiner, P., et al., J. Cell. Biol. 157: 1197-1209, 2002).)

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    WB

     WB: Use a concentration ...Read more →

    WB: Use a concentration of 0.5 - 2 µg/ml.Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).

Target

Function

Regulates endocytic recycling. May exert its functions by interacting with multiple effector proteins in different complexes. Acts as a major regulator of membrane delivery during cytokinesis. Together with MYO5B and RAB8A participates in epithelial cell polarization. Together with RAB3IP, RAB8A, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis (By similarity). Together with MYO5B participates in CFTR trafficking to the plasma membrane and TF (Transferrin) recycling in nonpolarized cells. Required in a complex with MYO5B and RAB11FIP2 for the transport of NPC1L1 to the plasma membrane. Participates in the sorting and basolateral transport of CDH1 from the Golgi apparatus to the plasma membrane. Regulates the recycling of FCGRT (receptor of Fc region of monomeric Ig G) to basolateral membranes.

Sequence similarities

Belongs to the small GTPase superfamily. Rab family.

Cellular localization

Cell membrane. Recycling endosome membrane. Cleavage furrow. Translocates with RAB11FIP2 from the vesicles of the endocytic recycling compartment (ERC) to the plasma membrane. Localizes to the cleavage furrow. Colocalizes with PARD3, PRKCI, EXOC5, OCLN, PODXL and RAB8A in apical membrane initiation sites (AMIS) during the generation of apical surface and luminogenesis.

Target information above from: UniProt accessionP62491 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • MGC1490 antibody
  • Rab 11 antibody
  • Rab 11A antibody
  • Rab-11 antibody
  • RAB11 A antibody
  • RAB11 antibody
  • Rab11a antibody
  • RAB11A member RAS oncogene family antibody
  • Ras related protein Rab 11A antibody
  • Ras related protein Rab11A antibody
  • Ras-related protein Rab-11A antibody
  • RB11A_HUMAN antibody
  • YL 8 antibody
  • YL8 antibody
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Database links

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Anti-Rab11A antibody [47] images:

  Immunocytochemistry/ Immunofluorescence - Rab11A antibody [47] (ab78337)

Immunocytochemistry/ Immunofluorescence - Rab11A antibody [47] (ab78337)

Ab78337, at 10ug/ml, staining HT1080 cells by Immunocytochemistry/ Immunofluorescence.

  Flow Cytometry-Rab11A antibody [47](ab78337)

Flow Cytometry-Rab11A antibody [47](ab78337)

Overlay histogram showing HEK293 cells stained with ab78337 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab78337, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a significantly decreased signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

References for Anti-Rab11A antibody [47] (ab78337)

This product has been referenced in:

  • Oehlke Oet al. Rab11b and its effector Rip11 regulate the acidosis-induced traffic of V-ATPase in salivary ducts. J Cell Physiol 226:638-51 (2011). WB, ICC/IF; Human.Read more (PubMed: 20717956) »
  • Amorim MJet al. A rab11- and microtubule-dependent mechanism for cytoplasmic transport of influenza a virus viral RNA. J Virol 85:4143-56 (2011).Read more (PubMed: 21307188) »

See all 6 publications for this product

Publishing research using ab78337? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"