Rab11A antibody [47] (ab78337)

Product Name 

Rab11A antibody [47]

Product type 

Primary antibodies

Description 

Mouse monoclonal [47] to Rab11A

Immunogen 

Recombinant fragment, corresponding to amino acids 86-207 of Human Rab11A

Reacts with 
(species key)

Hu, Ms, Rat, Chk, Dog, Mk

Tested applications 
(see key)

ICC/IF, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
WB: Use at a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
ICC/IF: Use at an assay dependent dilution.
This antibody has been reported by an independent laboratory to produce positive staining for Rab11A in ICC/IF using rat adrenal gland (Steiner, P., et al., J. Cell. Biol. 157: 1197-1209, 2002).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

HEK293 cell lysate. HeLa cell lysate. Rat adrenal gland.

Cellular localization 

Cell membrane; Peripheral membrane protein. Recycling endosome membrane; Peripheral membrane protein.

Research areas 

Cancer >> Signal transduction >> G protein signaling >> Small G proteins >> Ras family
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family

Relevance 

Rab proteins are low-molecular-weight GTP-binding proteins that form the largest branch of the Ras superfamily of GTPases. Located on the cytoplasmic face of organelles and vesicles, Rab proteins are involved in intracellular membrane fusion reactions. Three membrane proteins, synaptosomal associated protein of 25 kDa (SNAP-25), synaptobrevin, and syntaxin, form the core of a ubiquitous membrane fusion machine that interacts with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and a-SNAP. Rab proteins, in coordination with the core fusion machinery and Munc-18, help to mediate vesicle docking and fusion. There are over 40 Rab proteins in mammals. Rab11a and Rab11b are known markers for protein trafficking, sorting, and recycling in the endosomal pathway. The Rab11 proteins are enriched in recycling endosomes and the trans-Golgi network, where they regulate membrane recycling back to the plasma membrane. Rab11a is ubiquitously expressed, while Rab11b is expressed mainly in the heart and brain.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

47

Isotype 

IgG2a

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.09% Sodium Azide
Constituents: 30% Glycerol, PBS

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

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References for Rab11A antibody [47] (ab78337)

  This product has been used in: (two most recent references)

Kimura N et al. Dynein dysfunction induces endocytic pathology accompanied by an increase in Rab GTPases: a potential mechanism underlying age-dependent endocytic dysfunction. J Biol Chem 284:31291-302 (2009). ICC/IF; Monkey. PubMed: 19758999

This clone has been used in:
If you publish research using ab78337 please let us know so that we can cite the reference on this datasheet.

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