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Products:Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Overview
Product name
Anti-Rab11A antibody
See all Rab11A products (5) ...
Description
Rabbit polyclonal to Rab11A
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Sheep, Chicken, Cow, Rhesus monkey
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human Rab11.
(Peptide available as ab66706.)
Positive control
This antibody gave a positive signal in the following Human Whole Cell Lysates: HeLa, Jurkat, MCF7, SHSY-5Y
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Signal transduction >> G protein signaling >> Small G proteins >> Ras family
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Applications
Show applications key
Our Abpromise guarantee covers the use of ab65200 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
ICC/IF: Use a concentration of 1 µg/ml
Target
Function
Regulates endocytic recycling. May exert its functions by interacting with multiple effector proteins in different complexes. Acts as a major regulator of membrane delivery during cytokinesis. Together with MYO5B and RAB8A participates in epithelial cell polarization. Together with RAB3IP, RAB8A, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis (By similarity). Together with MYO5B participates in CFTR trafficking to the plasma membrane and TF (Transferrin) recycling in nonpolarized cells. Required in a complex with MYO5B and RAB11FIP2 for the transport of NPC1L1 to the plasma membrane. Participates in the sorting and basolateral transport of CDH1 from the Golgi apparatus to the plasma membrane. Regulates the recycling of FCGRT (receptor of Fc region of monomeric Ig G) to basolateral membranes.
Sequence similarities
Belongs to the small GTPase superfamily. Rab family.
Cellular localization
Cell membrane. Recycling endosome membrane. Cleavage furrow. Translocates with RAB11FIP2 from the vesicles of the endocytic recycling compartment (ERC) to the plasma membrane. Localizes to the cleavage furrow. Colocalizes with PARD3, PRKCI, EXOC5, OCLN, PODXL and RAB8A in apical membrane initiation sites (AMIS) during the generation of apical surface and luminogenesis.
Target information above from: UniProt accessionP62491
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- MGC1490 antibody
- Rab 11 antibody
- Rab 11A antibody
see all
Database links
- Entrez Gene: 415544 Chicken
- Entrez Gene: 8766 Human
- Entrez Gene: 53869 Mouse
- Omim: 605570 Human
- SwissProt: Q5ZJN2 Chicken
- SwissProt: P62491 Human
- SwissProt: P62492 Mouse
- Unigene: 321541 Human
see all
Anti-Rab11A antibody images:
Immunocytochemistry/ Immunofluorescence - Rab11 antibody (ab65200)
ICC/IF image of ab65200 stained MCF-7 cells. The cells were fixed with 4% PFA (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65200 at 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive result in 100% methanol (5 min) fixed MCF7 cells at 5µg/ml.
Western blot - Rab11 antibody (ab65200)
All lanes : Anti-Rab11A antibody (ab65200) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 4 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 24 kDa
Observed band size : 24 kDa
Additional bands at : 26 kDa (possible post-translational modification),45 kDa,72 kDa. We are unsure as to the identity of these extra bands.
We believe that the additional bands observed at 45 and 72 kDa may be a result of disulphide-linked forms of the Rab11 protein
References for Anti-Rab11A antibody (ab65200)
ab65200 has not yet been referenced specifically in any publications.
Publishing research using ab65200? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"