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Western blot - RAB11FIP5 antibody (ab68947)
(enlarge)
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Product Name
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RAB11FIP5 antibody
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Product type
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Primary antibodies
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Description
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Mouse polyclonal to RAB11FIP5
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Immunogen
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Full length protein, corresponding to amino acids 1-652 of Human RAB11FIP5
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/500 - 1/1000. Detects a band of approximately 77 kDa (predicted molecular weight: 70 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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RAB11FIP5 transfected 293T cell lysate.
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Cellular localization
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Cytoplasm. Recycling endosome membrane; Peripheral membrane protein. Mitochondrion membrane.
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Research areas
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Signal Transduction >> Protein Trafficking >> Organelle Proteins
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microtubules >> Tubulin
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Endosome
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Relevance
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RAB11FIP5 is enriched in polarized epithelial cells where it localises to subapical recycling endosomes (AREs) and the apical plasma membrane. It is a Rab effector involved in protein trafficking between these two locations. Autoantibodies are found to this protein in sera from patients with systemic lupus erythematosus (SLE) or Sjoegren syndrome (SS), and in the sera from mothers of children with neonatal lupus erythematosus (NLE).
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein G purified
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Storage buffer
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Preservative: None
Constituents: 1X PBS, pH 7.2
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this RAB11FIP5 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for RAB11FIP5 antibody images, references, products related to ab68947 and other tools.
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RAB11FIP5 antibody images:
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 Western blot - RAB11FIP5 antibody (ab68947)
All lanes : RAB11FIP5 antibody (ab68947) at 1/500 dilution
Lane 1 : RAB11FIP5 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 70 kDa
Observed band size : 77 kDa (why is the actual band size different from the predicted?)
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Search PubMed (MEDLINE) for references to RAB11FIP5
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RAB11FIP5 antibody - more information
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Customer reviews (feedback) regarding RAB11FIP5 antibody |
Customer FAQs regarding RAB11FIP5 antibody |
Protocols for RAB11FIP5 antibody |
Price and availability of products related to RAB11FIP5 antibody |
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RAB11FIP5 antibody - related products:
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RAB11FIP5 antibody - other tools:
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Contact Abcam with a Technical Enquiry about RAB11FIP5 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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