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Western blot - RAB13 antibody (ab37654)
(enlarge)
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Product Name
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RAB13 antibody
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See all RAB13 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Chicken polyclonal to RAB13
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Immunogen
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Immunogen corresponds to full length protein (Human)
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Reacts with
(species key)
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Hu, Ms, Rat
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/2000. Detects a band of approximately 75 kDa for the fusion protein (predicted molecular weight for the native protein: 23 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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E coli derived fusion protein.
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Cellular localization
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Cell Membrane; cell to cell junction; tight junction; lipid anchor; cytoplasmic side. Cytoplasmic vesicle; cytoplasmic vesicle membrane; lipid anchor; cytoplasmic side. Cytoplasmic tight junctions or associated with vesicles scattered throughout the cytoplasm in cells lacking tight junctions.
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Research areas
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Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Tight Junctions
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
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Relevance
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RAB13 may participate in polarized transport, in the assembly and/or the activity of tight junctions. Detected in several types of epithelia, including intestine, kidney, liver and in endothelial cells.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Chicken
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Clonality
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Polyclonal
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Isotype
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IgY
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: None
Constituents: PBS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this RAB13 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for RAB13 antibody images, references, products related to ab37654 and other tools.
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RAB13 antibody images:
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 Western blot - RAB13 antibody (ab37654)
RAB13 antibody (ab37654) at 1/2000 dilution + E coli derived fusion protein at 2.5 µg
Secondary
Goat anti IgY HRP at 1/1000 dilution
developed using the ECL technique
Predicted band size : 23 kDa
Observed band size : 75 kDa (why is the actual band size different from the predicted?)
The arrow next to the image points to the RAB13 fusion protien.
The use of a fusion protien may account for the difference observed in molecular weight compared to the native protien.
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Search PubMed (MEDLINE) for references to RAB13
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RAB13 antibody - more information
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Customer reviews (feedback) regarding RAB13 antibody |
Customer FAQs regarding RAB13 antibody |
Protocols for RAB13 antibody |
Price and availability of products related to RAB13 antibody |
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RAB13 antibody - related products:
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RAB13 antibody - other tools:
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Contact Abcam with a Technical Enquiry about RAB13 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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