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Western blot - RAB14 antibody (ab28639)
Western blot - RAB14 antibody (ab28639)
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Immunohistochemistry (Paraffin-embedded sections) - RAB14 antibody (ab28639)
Immunohistochemistry (Paraffin-embedded sections) - RAB14 antibody (ab28639)
(enlarge)


Product Name 

RAB14 antibody

 

See all RAB14 antibodies (2)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to RAB14

Immunogen 

Synthetic peptide derived from a region between amino acids 165-214 of human RAB14.
(Note: the exact amino acid sequence is proprietary).

Reacts with 
(species key)

Hu

Tested applications 
(see key)

ELISA, IHC-P, WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
ELISA: 1/12500.
IHC-P: Use at a concentration of 4-8 µg/ml.
WB: Use at a concentration of 0.5 µg/ml. Predicted molecular weight: 24 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Raji cell lysate, Human kidney tissue

Cellular localization 

Attached to the cytoplasmic side of the membrane by a lipid-anchor.

Research areas 

Signal Transduction >> Protein Trafficking >> Golgi Proteins
Signal Transduction >> Protein Trafficking >> Organelle Proteins

Relevance 

Rab14 is localized to biosynthetic and endosomal compartments, and is involved in the biosynthetic/recycling pathway between the Golgi and endosomal compartments.

 

RAB14 antibody (ab28639)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

51552    (Human)

SwissProt

    

P61106    (Human)

Unigene

    

371563    (Human)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

IgG fraction

Storage buffer 

Preservative: None
Constituents: 2% Sucrose, PBS

Purification notes 

Purified by peptide affinity chromatography method.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this RAB14 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for RAB14 antibody images, references, products related to ab28639 and other tools.

 

RAB14 antibody images:

Product image  Western blot - RAB14 antibody (ab28639)

Western blot - RAB14 antibody (ab28639)

RAB14 antibody (ab28639) at 0.5 µg/ml + Raji cell lysate 10ug.

Secondary
HRP conjugated anti-Rabbit IgG

Predicted band size : 24 kDa
Observed band size : 22 kDa (why is the actual band size different from the predicted?)

 

Product image  Immunohistochemistry (Paraffin-embedded sections) - RAB14 antibody (ab28639)

Immunohistochemistry (Paraffin-embedded sections) - RAB14 antibody (ab28639)

Human kidney tissue stained with ab28639 at X400 magnigication. The arrows point to epithelial cells of the renal tubule [positive label].

 

References for RAB14 antibody (ab28639)

Specific publication references   This product has been used in:

Ishikura S & Klip A Muscle cells engage Rab8A and myosin Vb in insulin-dependent GLUT4 translocation. Am J Physiol Cell Physiol 295:C1016-25 (2008). PubMed: 18701652

If you publish research using ab28639 please let us know so that we can cite the reference on this datasheet.

 

Search PubMed (MEDLINE) for references to RAB14

 

RAB14 antibody - more information

Customer reviews (feedback) regarding RAB14 antibody

Customer FAQs regarding RAB14 antibody

Protocols for RAB14 antibody

Price and availability of products related to RAB14 antibody

 

RAB14 antibody - related products:

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Loading controls

Lysates

 

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ab4347

  

Kidney (human) normal tissue slides

 

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RAB14 antibody - other tools:

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