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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RAB27A antibody (ab55667)
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Immunocytochemistry/ Immunofluorescence - RAB27A antibody (ab55667)
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Western blot - RAB27A antibody (ab55667)
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Product Name
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RAB27A antibody
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Product type
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Primary antibodies
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Description
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Mouse monoclonal to RAB27A
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Immunogen
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Recombinant fragment: YCENPDIVLC GNKSDLEDQR VVKEEEAIAL AEKYGIPYFE TSAANGTNIS QAIEMLLDLI MKRMERCVDK SWIPEGVVRS NGHASTDQLS EEKEKGACGC , corresponding to amino acids 122-222 of Human RAB27A
BLAST 'YCENPDIVLC GNKSDLEDQR VVKEEEAIAL AEKYGIPYFE TSAANGTNIS QAIEMLLDLI MKRMERCVDK SWIPEGVVRS NGHASTDQLS EEKEKGACGC ' with  or 
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Reacts with
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Hu
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Tested applications
(see key)
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ICC/IF, IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: 1/200.
IHC-P: Use at a concentration of 3 µg/ml.
IP: Use at an assay dependent dilution.(see Abreview)
WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 25 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Plasma membrane
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Research areas
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Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
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Relevance
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The protein encoded by this gene belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. It is thought that Rab27a may play a role in exocytosis. Mutations in this gene are associated with Griscelli syndrome type 2. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified (from Entrex Gene).
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Isotype
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IgG1
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Light chain type
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kappa
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Purity
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Protein G purified
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Storage buffer
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Preservative: None
PBS, pH 7.2
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this RAB27A antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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RAB27A antibody images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RAB27A antibody (ab55667)
RAB27A antibody (ab55667) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human lymphoma.
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Immunocytochemistry/ Immunofluorescence - RAB27A antibody (ab55667)
ab55667 staining cow Retinal Pigment Epithelium (RPE) cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.5% Triton X-100 prior to blocking in 10% serum for 20 minutes at 25°C. The primary antibody was diluted 1/200 and incubated with the sample for 12 hours at 4°C. An Alexa Fluor® 546 conjugated goat anti-mouse antibody was used as the secondary. Rab27a, which localizes to the apical site of the RPE cells, is labeled with red fluorescence. Nuclei were counterstained with DAPI (blue). This image is courtesy of an Abreview submitted by Dr Vladimir Milenkovic
See Abreview
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Western blot - RAB27A antibody (ab55667)
All lanes : RAB27A antibody (ab55667) at 1/200 dilution
Lane 1 : whole cell lysate of HEK 293 transfected with empty pCDNA3 vector
Lane 2 : whole cell lysate of HEK 293 cells expressing Rab27a-GFP
Lysates/proteins at 20 µg per lane.
Secondary
HRP conjugated goat anti-mouse
developed using the ECL technique
Predicted band size : 25 kDa
Observed band size : 52 kDa (why is the actual band size different from the predicted?)
Exposure time : 30 minutes
Rab27a (25kDa)+GFP(27kDa)=52kDa The blot was blocked with 5% milk for 30 minutes at 25°C and incubated with the primary antibody for 12 hours at 4°C. This image is courtesy of an Abreview submitted by Dr Vladimir Milenkovic
See Abreview
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Immunoprecipitation - RAB27A antibody (ab55667)
ab55667 (undiluted) was incubated with whole cell lysate of HEK 293 cells transfected with Rab27a-GFP and a Protein G matrix for 12 hours at 4°C to achieve immunoprecipitation. 20µg of lysate were present in the input. ab55667 (diluted 1/200) was also used in the western blotting step. Lane 1. lysate of HEK 293 cells expressing Rab27a-GFP
Lane 2. IP with ab55667
Lane 3. Not bound fraction This image is courtesy of an Abreview submitted by Dr Vladimir Milenkovic
See Abreview
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Search PubMed (MEDLINE) for references to RAB27A
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