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Anti-RAB40B antibody (ab74450)

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Overview

Product name

Anti-RAB40B antibody
See all RAB40B products (3) ...

Description

Rabbit polyclonal to RAB40B

Specificity

ab74450 detects endogenous levels of total RAB40B protein.

Tested applications

WB, ELISAmore details

Cross reactivity

Reacts with

Human

Immunogen

Synthetic peptide derived from an internal sequence of human RAB40B.

Positive control

Jurkat cell extracts.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

ab74450 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.

Clonality

Polyclonal

Isotype

IgG

Research areas

Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> SCF Complex E3 Ligase

  • Western blot - RAB40B antibody (ab74450)Western blot - RAB40B antibody (ab74450) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab74450 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/500 - 1/1000.Predi...Read more →

    WB: 1/500 - 1/1000.Predicted molecular weight: 31 kDa.

  • ELISA

     ELISA: 1/40000

    ELISA: 1/40000

Target

Relevance

RAB40B is similar to a yeast protein which suggests a role of the gene product in regulating secretory vesicles. It may be a substrate recognition component of a SCF like ECS (Elongin Cullin SOCS box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.

Cellular localization

Cell membrane; Lipid anchor; Cytoplasmic side

Alternative names

  • FLJ42385 antibody
  • SEC4L antibody
  • GTP binding protein homologous to Saccharomyces cerevisiae SEC4 antibody
  • RAB40B member RAS oncogene family antibody
  • RAR antibody
  • Rar protein antibody
  • Ras related protein Rab 40B antibody
  • RP23 293H17.2 antibody
  • SOCS box containing protein RAR antibody
see all

Database links

Anti-RAB40B antibody images:

  Western blot - RAB40B antibody (ab74450)

Western blot - RAB40B antibody (ab74450)

All lanes : Anti-RAB40B antibody (ab74450) at 1/500 dilution

Lane 1 : Jurkat cell extracts
Lane 2 : Jurkat cell extracts with immunising peptide at 10 µg

Lysates/proteins at 30 µg per lane.


Predicted band size : 31 kDa
Observed band size : 31 kDa

References for Anti-RAB40B antibody (ab74450)

ab74450 has not yet been referenced specifically in any publications.

Publishing research using ab74450? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"