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Products:Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Overview
Product name
Anti-RAB6A antibody
See all RAB6A products (3) ...
Description
Mouse monoclonal to RAB6A
Tested applications
WB, ICC/IF, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment: DDVRTERGSD VIIMLVGNKT DLADKRQVSI EEGERKAKEL NVMFIETSAK AGYNVKQLFR RVAAALPGME STQDRSREDM IDIKLEKPQE QPVSEGGCSC , corresponding to amino acids 109-208 of Human RAB6A
DDVRTERGSD VIIMLVGNKT DLADKRQVSI EEGERKAKEL NVMFIETSAK AGYNVKQLFR RVAAALPGME STQDRSREDM IDIKLEKPQE QPVSEGGCSC
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG2b
Light chain type
kappa
Research areas
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Applications
Show applications key
Our Abpromise guarantee covers the use of ab55660 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 5 µg/ml.Predicted molecular weight: 24 kDa.
ICC/IF: Use a concentration of 5 µg/ml
IHC-P: Use a concentration of 3 µg/ml
Target
Relevance
RAB6A belongs to the small GTPase superfamily. It may be involved in protein transport. It is a regulator of membrane traffic from the Golgi apparatus towards the endoplasmic reticulum (ER) and has a low GTPase activity.
Cellular localization
Golgi apparatus membrane; Lipid-anchor.
Alternative names
- GTP binding protein RAB6B antibody
- Oncogene RAB6 antibody
- Rab GTPase antibody
see all
Database links
- Entrez Gene: 5870 Human
- Omim: 179513 Human
- SwissProt: P20340 Human
Anti-RAB6A antibody images:
IHC-P - RAB6A antibody (ab55660)
RAB6A antibody (ab55660) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human tonsil.
Western blot - RAB6A antibody (ab55660)
RAB6A antibody (ab55660) at 1ug/lane + HL-60 cell lysate at 25ug/lane.
Immunocytochemistry/ Immunofluorescence - RAB6A antibody (ab55660)
ICC/IF image of ab55660 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55660, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-RAB6A antibody (ab55660)
ab55660 has not yet been referenced specifically in any publications.
Publishing research using ab55660? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"