Anti-Rab-6 antibody (ab95954)
Key features and details
- Rabbit polyclonal to Rab-6
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Rab-6 antibody -
Description
Rabbit polyclonal to Rab-6 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant full length protein corresponding to Human Rab-6.
Database link: P20340 -
Positive control
- WB: Transfected HeLa, A549, H1299, HCT 116 cells, mouse and rat brain tissue lysate; IHC-P: MDA-MB 468 xenograft tissue; IF: A549 cells.
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General notes
We have not tested the cross reactivity to RAB6B. ab95954 is raised against a full length RAB6A recombinant protein and may cross-react with RAB6B.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab95954 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (3) |
1/500 - 1/3000. Predicted molecular weight: 24 kDa.
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IHC-P |
1/100 - 1/500.
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ICC/IF | (3) |
1/100 - 1/200.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 24 kDa. |
IHC-P
1/100 - 1/500. |
ICC/IF
1/100 - 1/200. |
Target
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Function
Protein transport. Regulator of membrane traffic from the Golgi apparatus towards the endoplasmic reticulum (ER). Has a low GTPase activity. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the small GTPase superfamily. Rab family. -
Cellular localization
Golgi apparatus membrane. - Information by UniProt
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Database links
- Entrez Gene: 5870 Human
- Entrez Gene: 19346 Mouse
- Entrez Gene: 84379 Rat
- Omim: 179513 Human
- SwissProt: P20340 Human
- SwissProt: P35279 Mouse
- SwissProt: Q9WVB1 Rat
- Unigene: 503222 Human
see all -
Alternative names
- GTP binding protein RAB6A' antibody
- GTP binding protein RAB6B antibody
- Oncogene RAB6 antibody
see all
Images
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All lanes : Anti-Rab-6 antibody (ab95954) at 1/1000 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : H1299 (human non-small cell lung carcinoma cell line) whole cell lysate
Lane 3 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 24 kDa12 % SDS-PAGE
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Immunohistochemical analysis of paraffin-embedded MDA-MB 468 xenograft tissue staining Rab-6 with ab95954 at 1:500 dilution.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.
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Immunofluorescence analysis of 4% paraformaldehyde-fixed A549 (human lung carcinoma cell line) whole cell labling Rab-6 protein at Golgi apparatus with ab95954 at 1:500 dilution. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
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All lanes : Anti-Rab-6 antibody (ab95954) at 1/500 dilution
Lane 1 : Wild-type HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : Rab-6 knockout HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 24 kDa12% SDS-PAGE
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Anti-Rab-6 antibody (ab95954) at 1/1000 dilution + Rat brain tissue lysate at 50 µg
Predicted band size: 24 kDa12 % SDS-PAGE
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Anti-Rab-6 antibody (ab95954) at 1/1000 dilution + Mouse brain tissue lysate at 50 µg
Predicted band size: 24 kDa12 % SDS-PAGE
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab95954 has been referenced in 6 publications.
- Fujii S et al. Recycling endosomes attach to the trans-side of Golgi stacks in Drosophila and mammalian cells. J Cell Sci 133:N/A (2020). PubMed: 31974113
- Akin EJ et al. Building sensory axons: Delivery and distribution of NaV1.7 channels and effects of inflammatory mediators. Sci Adv 5:eaax4755 (2019). PubMed: 31681845
- Abdullah MI et al. Inhibition of the mevalonate pathway augments the activity of pitavastatin against ovarian cancer cells. Sci Rep 7:8090 (2017). PubMed: 28808351
- Hou X et al. Rab6a is a novel regulator of meiotic apparatus and maturational progression in mouse oocytes. Sci Rep 6:22209 (2016). PubMed: 26915694
- Petrosyan A et al. Restoration of compact Golgi morphology in advanced prostate cancer enhances susceptibility to galectin-1-induced apoptosis by modifying mucin O-glycan synthesis. Mol Cancer Res 12:1704-16 (2014). PubMed: 25086069
- Petrosyan A & Cheng PW A non-enzymatic function of Golgi glycosyltransferases: mediation of Golgi fragmentation by interaction with non-muscle myosin IIA. Glycobiology 23:690-708 (2013). PubMed: 23396488