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Products:Tags & Cell Markers >> Subcellular Markers >> Organelles >> Endosome
Overview
Product name
Anti-RAB7 antibody
See all RAB7 products (5) ...
Description
Rabbit polyclonal to RAB7
Specificity
ab74906 is specific for RAB7.
Tested applications
WB, IHC-P, IHC-Frmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Xenopus laevis
Immunogen
Synthetic peptide from the internal part of human RAB7 conjugated to an immunogenic carrier protein.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C (add glycerol to a final volume of 40% for extra stability). Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS, Whole serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Lysosome
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Endosome
Applications
Show applications key
Our Abpromise guarantee covers the use of ab74906 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
IHC-P: 1/300 - 1/2000. Antigen retrieval recommended.
IHC-Fr: 1/300 - 1/2000.
WB: 1/300 - 1/2000. Predicted molecular weight: 23 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Members of the RAB family of RAS related GTP binding proteins are important regulators of vesicular transport and are located in specific intracellular compartments. RAB7 has been localized to late endosomes and shown to be important in the late endocytic pathway. In addition, it has been shown to have a fundamental role in the cellular vacuolation induced by the cytotoxin VacA of Helicobacter pylori.
Cellular localization
Endosome; late endosome. Lysosome. Cytoplasmic vesicle; phagosome. Melanosome.
Alternative names
- MGC16212 antibody
- CMT2B antibody
- Member RAS oncogene family antibody
see all
Database links
- Entrez Gene: 7879 Human
- Entrez Gene: 19349 Mouse
- Entrez Gene: 446841 Xenopus laevis
- Omim: 602298 Human
- SwissProt: P51149 Human
- SwissProt: P51150 Mouse
Anti-RAB7 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-RAB7 antibody(ab74906)
ab74906 (2µg/ml) staining Rab 7 in human stomach using an automated system (DAKO Autostainer Plus). Using this protocol there is cytoplasmic staining of gastric mucosa.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Western blot - RAB7 antibody (ab74906)
All lanes : Anti-RAB7 antibody (ab74906) at 1/500 dilution
Lane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)
Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 23 kDa
Observed band size : 23 kDa
Additional bands at : 74 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 1 minute
References for Anti-RAB7 antibody (ab74906)
ab74906 has not yet been referenced specifically in any publications.
Publishing research using ab74906? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"