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Product name
Anti-RAC1 + Cdc42 (phospho S71) antibody
See all RAC1 + Cdc42 products (4) ...
Description
Rabbit polyclonal to RAC1 + Cdc42 (phospho S71)
Specificity
Cdc 42 [pS71] (100% homologous) and Rho A/B/C [pS73] (92% homologous) are expected to react.
Tested applications
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic phospho peptide (Human) containing serine 71. The sequence is conserved in human and mouse RAC 1, 2, and 3, and Cdc 42 human, mouse, rat, dog and frog.
Positive control
A431 cells treated with EGF.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 1mg/ml BSA (IgG, protease free). pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated RAC 1. The final product is generated by affinity chromatography using a RAC 1 derived peptide that is phosphorylated at serine 71.
Primary antibody notes
RAC, Cdc 42 and Rho A, B, and C are members of a small RhoGTPase family that bind and hydrolyze GTP. GTP bound RAC 1 and cdc 42 play a pivotal role in controlling cell shape, adhesion, growth and transformation. Active Rac 1 is implicated in regulating serum response element (SRE), NFAT 1 and nuclear factor kappa B (NF kappa B) transcription activities. Activated RAC 1 and Cdc 42 bind and activate PAK 1, which in turn activates key downstream signaling proteins including MEKK 1 and JNK. RAC 1 and Cdc 42 are phosphorylated on serine 71, a putative Akt site located between the protein binding domain and GTP binding domain. Phosphorylation of RAC 1 on serine 71 regulates its GTP binding and GTPase activity.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Other
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Assembly
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Other
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- RAC1 + Cdc42 (phospho S71) antibody (ab5482)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab5482 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000. Detects a band of approximately 23 kDa.
Target
Relevance
Cdc42/Rac belongs to the superfamily of small GTPases that are structurally linked to the proto-oncogene product p21ras and are important for the control of cell growth and differentiation as well as for intracellular organization. Cdc42/Rac is an important upstream regulator of the protein kinase cascade that controls the SAPK/JNK and p38 activity. Recent data also suggest that constitutive active forms of Cdc42 can induce apoptosis through a mechanism requiring signaling through SAPK/JNK.
Cellular localization
Cell membrane; Lipid anchor; Cytoplasmic side.
Alternative names
- Biliverdin reductase B antibody
- Biliverdin-IX beta-reductase antibody
- BVRB antibody
see all
Database links
- Entrez Gene: 5879 Human
- Entrez Gene: 998 Human
- Entrez Gene: 19353 Mouse
- Entrez Gene: 12540 Mouse
- Entrez Gene: 363875 Rat
- Entrez Gene: 64465 Rat
- Omim: 602048 Human
- Omim: 116952 Human
see all
Anti-RAC1 + Cdc42 (phospho S71) antibody images:
- RAC1 + Cdc42 (phospho S71) antibody (ab5482)
Peptide Competition and Phosphatase Treatment:
Lysates prepared from A431 cells left unstimulated (1) or stimulated with EGF (2-6) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either not treated (1-5) or treated with lambda phosphatase (6), blocked with a 5% BSA-TBST buffer for one hour at room temperature, and incubated with ab5482 antibody for two hours at room temperature in a 3% BSATBST buffer, following prior incubation with: no peptide (1, 2, 6), the nonphosphopeptide corresponding to the immunogen (3), a generic phosphoserine containing peptide (4), or, the phosphopeptide immunogen (5), After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal method. The data show that the peptide corresponding to ab5482 blocks the antibody signal. The data also shows that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific.
References for Anti-RAC1 + Cdc42 (phospho S71) antibody (ab5482)
This product has been referenced in:
- Haas S et al. DIGE proteome analysis reveals suitability of ischemic cardiac in vitro model for studying cellular response to acute ischemia and regeneration. PLoS One 7:e31669 (2012). WB; Mouse.Read more (PubMed: 22384053) »
- Pickl M & Ries CH Comparison of 3D and 2D tumor models reveals enhanced HER2 activation in 3D associated with an increased response to trastuzumab. Oncogene 28:461-8 (2009). WB; Human.Read more (PubMed: 18978815) »
See all 3 publications for this product
Publishing research using ab5482? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"