|
|
|
|
Immunohistochemistry (Frozen sections) - RAC1 + RAC2 antibody (ab68828)
(enlarge)
|
|
Immunohistochemistry (Frozen sections) - RAC1 + RAC2 antibody (ab68828)
(enlarge)
|
|
Immunohistochemistry (Frozen sections) - RAC1 + RAC2 antibody (ab68828)
(enlarge)
|
|
|
|
|
Product Name
|
RAC1 + RAC2 antibody
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to RAC1 + RAC2
|
|
Immunogen
|
Synthetic peptide (Human), conjugated to an immunogenic carrier protein
|
|
Reacts with
|
Hu, Ms, Rat
|
|
Predicted to react
(species key)
|
Chk, Cow, Dog, Gpig, Xl, Zfsh
(Due to sequence homology)
|
|
Tested applications
(see key)
|
IHC-Fr, IHC-P, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
IHC-P: 1/300 - 1/3000.
IHC-Fr: 1/300 - 1/3000.
WB: Use at an assay dependent concentration.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
Spinal cord.
|
|
Cellular localization
|
RAC1: Cell membrane; Lipid-anchor; Cytoplasmic side. Melanosome.
Note: Inner surface of plasma membrane possibly with attachment requiring prenylation of the C-terminal cysteine. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
RAC2: Cytoplasm.
Note: Membrane-associated when activated.
|
|
Research areas
|
Cancer >> Signal transduction >> G protein signaling >> Small G proteins >> Other
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Other
Cell Biology >> Apoptosis >> Intracellular >> Kinases
|
|
Relevance
|
RAC1 and RAC2 belong to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases.
They are plasma membrane-associated small GTPase which binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Whole antiserum
|
|
Storage buffer
|
Preservative: None
Constituents: Whole serum
|
|
Form
|
Liquid
|
|
Concentration
|
Concentration not determined - why is this?
|
|
Storage instructions
|
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C (add glycerol to a final volume of 40% for extra stability). Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this RAC1 + RAC2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for RAC1 + RAC2 antibody images, references, products related to ab68828 and other tools.
|
| |
RAC1 + RAC2 antibody images:
|
 Immunohistochemistry (Frozen sections) - RAC1 + RAC2 antibody (ab68828)
IHC on rat spinal cord (ventral horn) using ab68828 at 1 : 1000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).
|
| |
Immunohistochemistry (Frozen sections) - RAC1 + RAC2 antibody (ab68828)
IHC on rat ligated sciatic nerve using ab68828 at 1 : 1000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).
|
| |
Immunohistochemistry (Frozen sections) - RAC1 + RAC2 antibody (ab68828)
IHC on rat sciatic nerve using ab68828 at 1 : 1000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).
|
| |
| |
|
Search PubMed (MEDLINE) for references to RAC1 + RAC2
|
| |
RAC1 + RAC2 antibody - more information
|
Customer reviews (feedback) regarding RAC1 + RAC2 antibody |
Customer FAQs regarding RAC1 + RAC2 antibody |
Protocols for RAC1 + RAC2 antibody |
Price and availability of products related to RAC1 + RAC2 antibody |
| |
RAC1 + RAC2 antibody - related products:
|
|
|
| |
RAC1 + RAC2 antibody - other tools:
|
Contact Abcam with a Technical Enquiry about RAC1 + RAC2 antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
