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Products:Cell Biology >> Apoptosis >> Intracellular >> Kinases
Overview
Product name
Anti-RAC1 + RAC2 antibody
Description
Rabbit polyclonal to RAC1 + RAC2
Tested applications
WB, IHC-P, IHC-Frmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Chicken, Guinea pig, Cow, Dog, Xenopus laevis, Zebrafish
Immunogen
Synthetic peptide (Human), conjugated to an immunogenic carrier protein
Positive control
Spinal cord.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C (add glycerol to a final volume of 40% for extra stability). Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: Whole serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Signal transduction >> G protein signaling >> Small G proteins >> Other
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Other
Cell Biology >> Apoptosis >> Intracellular >> Kinases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab68828 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent concentration.
IHC-P
IHC-P: 1/300 - 1/3000.IHC-P: 1/300 - 1/3000.
IHC-Fr: 1/300 - 1/3000.
Target
Relevance
RAC1 and RAC2 belong to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. They are plasma membrane-associated small GTPase which binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles.
Cellular localization
RAC1: Cell membrane; Lipid-anchor; Cytoplasmic side. Melanosome. Note: Inner surface of plasma membrane possibly with attachment requiring prenylation of the C-terminal cysteine. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. RAC2: Cytoplasm. Note: Membrane-associated when activated.
Alternative names
- MIG5 antibody
- p21 Rac1 antibody
- p21 Rac2 antibody
see all
Database links
- Entrez Gene: 5879 Human
- Entrez Gene: 5880 Human
- Entrez Gene: 19353 Mouse
- Entrez Gene: 19354 Mouse
- Entrez Gene: 363875 Rat
- Entrez Gene: 366957 Rat
- Omim: 602048 Human
- Omim: 602049 Human
see all
Anti-RAC1 + RAC2 antibody images:
Immunohistochemistry (Frozen sections) - RAC1 + RAC2 antibody (ab68828)
IHC on rat spinal cord (ventral horn) using ab68828 at 1 : 1000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).
Immunohistochemistry (Frozen sections) - RAC1 + RAC2 antibody (ab68828)
IHC on rat ligated sciatic nerve using ab68828 at 1 : 1000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).
Immunohistochemistry (Frozen sections) - RAC1 + RAC2 antibody (ab68828)
IHC on rat sciatic nerve using ab68828 at 1 : 1000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).
References for Anti-RAC1 + RAC2 antibody (ab68828)
ab68828 has not yet been referenced specifically in any publications.
Publishing research using ab68828? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"