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Products:Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Other
Overview
Product name
Anti-RAC2 antibody
See all RAC2 products (3) ...
Description
Goat polyclonal to RAC2
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide: PQPTRQQKRACSLL, corresponding to C terminal amino acids 179-192 of Human RAC2.
PQPTRQQKRA CSLL
Positive control
MOLT4 or liver lysate.
General notes
Principal Names - RAC2; ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2); EN-7; Ras-related C3 botulinum toxin substrate 2; rho family, small GTP binding protein Rac2; Ras-related C3 botulinum toxin substrate 3 (rho family, small GTP); Gx; HSPC022.
Official Gene Symbol - RAC2.
GenBank Accession Number – NP_002863.
LocusLink ID - 5880.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab2244 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.1 - 1 µg/ml.Predicted molecular weight: 21 kDa.
IHC-P: Use a concentration of 0.3 µg/mlPerform heat mediated antigen retrieval before commencing with IHC staining protocol.(In Human Tonsil most cells in the mantle zone and interfollicular area are stained whilst those in the germinal centres remain negative.)
Target
Function
Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as secretory processes, phagocytose of apoptotic cells and epithelial cell polarization. Augments the production of reactive oxygen species (ROS) by NADPH oxidase.
Tissue specificity
Hematopoietic specific.
Involvement in disease
Defects in RAC2 are the cause of neutrophil immunodeficiency syndrome (NEUID) [MIM:608203].
Sequence similarities
Belongs to the small GTPase superfamily. Rho family.
Cellular localization
Cytoplasm. Membrane-associated when activated.
Target information above from: UniProt accessionP15153
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- EN 7 antibody
- EN7 antibody
- GX antibody
see all
Database links
- Entrez Gene: 5880 Human
- Entrez Gene: 19354 Mouse
- Omim: 602049 Human
- SwissProt: P15153 Human
- SwissProt: Q05144 Mouse
- Unigene: 517601 Human
- Unigene: 1972 Mouse
Anti-RAC2 antibody images:
Western blot - RAC2 antibody (ab2244)
Anti-RAC2 antibody (ab2244) at 0.3 µg/ml + Human Tonsil lysate(RIPA buffer, 35ug total protein per lane).
Predicted band size : 21 kDa
Observed band size : 20 kDa (why is the actual band size different from the predicted?)
Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RAC2 antibody (ab2244)
Ab2244 (0.3µg/ml) staining of paraffin embedded Human Tonsil. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. A) Strong staining in the interfollicular area and mantle zone but the majority of cells in germinal centre are negative. B) Higher magnification of interfollicular area showing positive cells.
References for Anti-RAC2 antibody (ab2244)
This product has been referenced in:
- Tomilov AAet al. Decreased superoxide production in macrophages of long-lived p66Shc knock-out mice. J Biol Chem 285:1153-65 (2010). WB; Mouse.Read more (PubMed: 19892704) »
- Ramgolam VSet al. T cell LFA-1 engagement induces HuR-dependent cytokine mRNA stabilization through a Vav-1, Rac1/2, p38MAPK and MKK3 signaling cascade. PLoS One 5:e14450 (2010). WB; Human.Read more (PubMed: 21206905) »
See all 2 publications for this product
Publishing research using ab2244? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"