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Western blot - RAC2 antibody (ab2244)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RAC2 antibody (ab2244)
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Product Name
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RAC2 antibody
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Product type
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Primary antibodies
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Description
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Goat polyclonal to RAC2
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Immunogen
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Synthetic peptide: PQPTRQQKRACSLL, corresponding to C terminal amino acids 179-192 of Human RAC2.
BLAST 'PQPTRQQKRACSLL' with  or 
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ELISA, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Peptide ELISA: Antibody detection limit dilution 1:32,000.
IHC-P: Use at a concentration of 0.3 µg/ml. In paraffin embedded Human Tonsil, stains most cells in the mantle zone and interfollicular area. In the germinal centres the majority of cells are negative.
WB: Use at a concentration of 0.3 - 3 µg/ml. Predicted molecular weight: 21 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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MOLT4 or liver lysate.
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Cellular localization
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Cytoplasm. Membrane-associated when activated.
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Research areas
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Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Other
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Relevance
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RAC2 is a small signaling G protein (more specifically a GTPase), and is a member of the RAC subfamily of the family Rho family of GTPases. RAC proteins play important roles in multiple cellular events, including actin cytoskeletal organization, cell proliferation and survival, cell cycle progression, and gene transcription regulation.
RAC1 and RAC2 are also essential components of NADPH oxidase, the enzyme responsible for generating free radicals.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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IgG fraction
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Storage buffer
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Tris saline, 0.02% Na azide, pH7.3 with 0.5% BSA Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Notes
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Principal Names - RAC2; ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2); EN-7; Ras-related C3 botulinum toxin substrate 2; rho family, small GTP binding protein Rac2; Ras-related C3 botulinum toxin substrate 3 (rho family, small GTP); Gx; HSPC022.
Official Gene Symbol - RAC2.
GenBank Accession Number – NP_002863.
LocusLink ID - 5880.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this RAC2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for RAC2 antibody images, references, products related to ab2244 and other tools.
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RAC2 antibody images:
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 Western blot - RAC2 antibody (ab2244)
RAC2 antibody (ab2244) at 2 µg/ml + MOLT-4 lysate (RIPA buffer, 35ug total protein per lane).
Predicted band size : 21 kDa
Observed band size : 22 kDa (why is the actual band size different from the predicted?)
Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RAC2 antibody (ab2244)
Ab2244 (0.3μg/ml) staining of paraffin embedded Human Tonsil. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. A) Strong staining in the interfollicular area and mantle zone but the majority of cells in germinal centre are negative. B) Higher magnification of interfollicular area showing positive cells.
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References for RAC2 antibody (ab2244)
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This product has been used in:
Tomilov AA et al. Decreased superoxide production in macrophages of long-lived p66Shc knock-out mice. J Biol Chem 285:1153-65 (2010). WB; Mouse. PubMed: 19892704
If you publish research using ab2244 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to RAC2
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RAC2 antibody - more information
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Customer reviews (feedback) regarding RAC2 antibody |
Customer FAQs regarding RAC2 antibody |
Protocols for RAC2 antibody |
Price and availability of products related to RAC2 antibody |
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RAC2 antibody - related products:
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RAC2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about RAC2 antibody |
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