Anti-RACK1 antibody (ab166796)
Key features and details
- Goat polyclonal to RACK1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
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- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-RACK1 antibody
See all RACK1 primary antibodies -
Description
Goat polyclonal to RACK1 -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rabbit, Horse, Chicken, Hamster, Cow, Dog, Pig, Monkey, Gorilla -
Immunogen
Synthetic peptide corresponding to internal sequence amino acids 133-147 of Human RACK1 (NP_006089.1).
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Positive control
- Human Small Intestine tissue; Human Lymph Node lysate; Mouse and Rat Spleen lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab166796 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 35 kDa).
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IHC-P |
Use a concentration of 3.75 µg/ml.
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Notes |
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WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 35 kDa). |
IHC-P
Use a concentration of 3.75 µg/ml. |
Target
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Function
Seems to bind protein kinase C acting as an intracellular receptor to anchor the activated PKC to the cytoskeleton. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and ITGB1, serves as a platform for SRC activation or inactivation. May play an important role in the developing brain and neuronal differentiation. -
Sequence similarities
Belongs to the WD repeat G protein beta family.
Contains 7 WD repeats. -
Domain
The WD repeats domain 5 mediates interaction with TRIM63. -
Cellular localization
Cell membrane. Located on plasma membrane of neuroblastoma NMB7 cells. - Information by UniProt
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Database links
- Entrez Gene: 417044 Chicken
- Entrez Gene: 327682 Cow
- Entrez Gene: 480818 Dog
- Entrez Gene: 100057771 Horse
- Entrez Gene: 10399 Human
- Entrez Gene: 397605 Pig
- Entrez Gene: 100009557 Rabbit
- Omim: 176981 Human
see all -
Alternative names
- Cell proliferation-inducing gene 21 protein antibody
- GBLP_HUMAN antibody
- Gnb2-rs1 antibody
see all
Images
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human small intestine tissue labeling RACK1 with ab166796 at 3.75 µg/ml.
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Anti-RACK1 antibody (ab166796) at 0.1 µg/ml + Human Lymph Node lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 35 kDa
Observed band size: 33 kDa why is the actual band size different from the predicted?
Primary incubation: 1 hour.
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab166796 has been referenced in 1 publication.
- Filho EGF et al. RACK1 plays a critical role in mast cell secretion and Ca2+ mobilization by modulating F-actin dynamics. J Cell Sci 134:N/A (2021). PubMed: 34550354