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Products:Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Cdk Regulators
Overview
Product name
Anti-RACK1 antibody
See all RACK1 products (8) ...
Description
Rabbit polyclonal to RACK1
Specificity
Ab27982 recognises RACK1.
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Recombinant full length RACK1protein (Human).
Positive control
Jurkat and NIH3T3 cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Thimerosal (merthiolate).
Constituents: 50% Glycerol, 1% BSA, PBS.
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Other Antibodies >> Other Antibodies
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKC
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Cdk Regulators
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Immunocytochemistry/ Immunofluorescence - Anti-RACK1 antibody (ab27982)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab27982 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 4 µg/ml.Predicted molecular weight: 35 kDa.
ICC/IF: Use a concentration of 5 µg/ml
Target
Function
Seems to bind protein kinase C acting as an intracellular receptor to anchor the activated PKC to the cytoskeleton. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and ITGB1, serves as a platform for SRC activation or inactivation. May play an important role in the developing brain and neuronal differentiation.
Sequence similarities
Belongs to the WD repeat G protein beta family.
Contains 7 WD repeats.
Domain
The WD repeats domain 5 mediates interaction with TRIM63.
Cellular localization
Cell membrane. Located on plasma membrane of neuroblastoma NMB7 cells.
Target information above from: UniProt accessionP63244
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Cell proliferation-inducing gene 21 protein antibody
- GBLP_HUMAN antibody
- Gnb2 rs1 antibody
see all
Database links
- Entrez Gene: 10399 Human
- Entrez Gene: 14694 Mouse
- Entrez Gene: 83427 Rat
- Omim: 176981 Human
- SwissProt: P63244 Human
- SwissProt: P68040 Mouse
- SwissProt: P63245 Rat
- Unigene: 5662 Human
see all
Anti-RACK1 antibody images:
Immunocytochemistry/ Immunofluorescence - Anti-RACK1 antibody (ab27982)
ICC/IF image of ab27982 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab27982, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-RACK1 antibody (ab27982)
ab27982 has not yet been referenced specifically in any publications.
Publishing research using ab27982? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"