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Products:Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Cdk Regulators
Overview
Product name
Anti-RACK1 antibody
See all RACK1 products (8) ...
Description
Rabbit polyclonal to RACK1
Tested applications
ELISA, WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide: TEQMTLRGTL KGHNGWVTQI ATTPQFPDMI LSASRDKTII MWKLTRDETN YGIPQRALRG HSHFVSDVVI SSDGQFALSG SWDGTLRLWD LTTGTTTRRF conjugated to KLH, corresponding to N terminal amino acids 1-100 of Human RACK1
TEQMTLRGTL KGHNGWVTQI ATTPQFPDMI LSASRDKTII MWKLTRDETN YGIPQRALRG HSHFVSDVVI SSDGQFALSG SWDGTLRLWD LTTGTTTRRF
Positive control
Ramos cell lysate. Hepatocarcinoma formalin-fixed, paraffin embedded tissue.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Other Antibodies >> Other Antibodies
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKC
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Cdk Regulators
Applications
Show applications key
Our Abpromise guarantee covers the use of ab37672 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/1000ELISA: 1/1000
WB: 1/100 - 1/500.Predicted molecular weight: 35 kDa.
IHC-P: 1/50 - 1/100.
Target
Function
Seems to bind protein kinase C acting as an intracellular receptor to anchor the activated PKC to the cytoskeleton. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and ITGB1, serves as a platform for SRC activation or inactivation. May play an important role in the developing brain and neuronal differentiation.
Sequence similarities
Belongs to the WD repeat G protein beta family.
Contains 7 WD repeats.
Domain
The WD repeats domain 5 mediates interaction with TRIM63.
Cellular localization
Cell membrane. Located on plasma membrane of neuroblastoma NMB7 cells.
Target information above from: UniProt accessionP63244
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Cell proliferation-inducing gene 21 protein antibody
- GBLP_HUMAN antibody
- Gnb2 rs1 antibody
see all
Database links
- Entrez Gene: 10399 Human
- Omim: 176981 Human
- SwissProt: P63244 Human
- Unigene: 5662 Human
Anti-RACK1 antibody images:
Western blot - RACK1 antibody (ab37672)
Anti-RACK1 antibody (ab37672) at 1/100 dilution + Ramos cell lysate.
Predicted band size : 35 kDa
Observed band size : 30 kDa (why is the actual band size different from the predicted?)
Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RACK1 antibody (ab37672)
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ab37672, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
References for Anti-RACK1 antibody (ab37672)
ab37672 has not yet been referenced specifically in any publications.
Publishing research using ab37672? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"