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Western blot - RACK1 antibody (ab37672)
(enlarge)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RACK1 antibody (ab37672)
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Product Name
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RACK1 antibody
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See all RACK1 antibodies (8)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to RACK1
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Immunogen
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Synthetic peptide: TEQMTLRGTL KGHNGWVTQI ATTPQFPDMI LSASRDKTII MWKLTRDETN YGIPQRALRG HSHFVSDVVI SSDGQFALSG SWDGTLRLWD LTTGTTTRRF conjugated to KLH, corresponding to N terminal amino acids 1-100 of Human RACK1
BLAST 'TEQMTLRGTL KGHNGWVTQI ATTPQFPDMI LSASRDKTII MWKLTRDETN YGIPQRALRG HSHFVSDVVI SSDGQFALSG SWDGTLRLWD LTTGTTTRRF ' with  or 
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ELISA, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/1000.
IHC-P: 1/50 - 1/100.
WB: 1/100 - 1/500. Predicted molecular weight: 35 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Ramos cell lysate.
Hepatocarcinoma formalin-fixed, paraffin embedded tissue.
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Cellular localization
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Cytoplasmic
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Research areas
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Cell Biology >> Other Antibodies >> Other Antibodies
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKC
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Cdk Regulators
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Relevance
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RACK1 (receptor for activated C kinase 1) was identified through its binding to various PKC isoforms. Its main function is to recruit PKC and various other proteins to specific location to form multiprotein complexes, mediating various signal pathways.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein G purified
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Storage buffer
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Preservative: 0.09% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.250 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this RACK1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for RACK1 antibody images, references, products related to ab37672 and other tools.
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RACK1 antibody images:
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 Western blot - RACK1 antibody (ab37672)
RACK1 antibody (ab37672) at 1/100 dilution + Ramos cell lysate.
Predicted band size : 35 kDa
Observed band size : 30 kDa (why is the actual band size different from the predicted?)
Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RACK1 antibody (ab37672)
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ab37672, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
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Search PubMed (MEDLINE) for references to RACK1
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RACK1 antibody - more information
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Customer reviews (feedback) regarding RACK1 antibody |
Customer FAQs regarding RACK1 antibody |
Protocols for RACK1 antibody |
Price and availability of products related to RACK1 antibody |
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RACK1 antibody - related products:
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RACK1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about RACK1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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