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Products:Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Cdk Regulators
Overview
Product name
Anti-RACK1 antibody
See all RACK1 products (8) ...
Description
Rabbit polyclonal to RACK1
Tested applications
Cross reactivity
Reacts with
Mouse, Rat, Chicken, Cow, Human, Pig
Immunogen
Synthetic peptide mapping to the C-terminus of human RACK1.
Positive control
Jurkat cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 30% Glycerol, 0.5% BSA, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Other Antibodies >> Other Antibodies
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKC
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Cdk Regulators
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Western blot - RACK1 antibody (ab59745)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab59745 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.5 - 4 µg/ml.Detects a band of approximately 34 kDa (predicted molecular weight: 35 kDa).
Target
Function
Seems to bind protein kinase C acting as an intracellular receptor to anchor the activated PKC to the cytoskeleton. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and ITGB1, serves as a platform for SRC activation or inactivation. May play an important role in the developing brain and neuronal differentiation.
Sequence similarities
Belongs to the WD repeat G protein beta family.
Contains 7 WD repeats.
Domain
The WD repeats domain 5 mediates interaction with TRIM63.
Cellular localization
Cell membrane. Located on plasma membrane of neuroblastoma NMB7 cells.
Target information above from: UniProt accessionP63244
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Cell proliferation-inducing gene 21 protein antibody
- GBLP_HUMAN antibody
- Gnb2 rs1 antibody
see all
Database links
- Entrez Gene: 417044 Chicken
- Entrez Gene: 327682 Cow
- Entrez Gene: 10399 Human
- Entrez Gene: 14694 Mouse
- Entrez Gene: 397605 Pig
- Entrez Gene: 83427 Rat
- Omim: 176981 Human
- SwissProt: P63247 Chicken
see all
Anti-RACK1 antibody images:
Western blot - RACK1 antibody (ab59745)
All lanes : Anti-RACK1 antibody (ab59745) at 0.5 µg/ml
Lane 1 : Jurkat cell lysate
Lane 2 : Jurket cell lysate
Predicted band size : 35 kDa
Observed band size : 34 kDa (why is the actual band size different from the predicted?)
References for Anti-RACK1 antibody (ab59745)
ab59745 has not yet been referenced specifically in any publications.
Publishing research using ab59745? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"