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Anti-RACK1 antibody (ab62735)

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Overview

Product name

Anti-RACK1 antibody
See all RACK1 products (8) ...

Description

Rabbit polyclonal to RACK1

Tested applications

WB, IP, ICC/IFmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Predicted to work with

Chicken, Cow, Pig, Xenopus laevis, Cynomolgus Monkey

Immunogen

Synthetic peptide: TEQMTLRGT LKGHNG - C, conjugated to KLH by a C terminal Cysteine residue linker, corresponding to N terminal amino acids 2-16 of Human RACK1

TEQMTLRGT LKGHNG - C

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

Paraformaldehyde-fixed HeLa cells, HEK-293T cell lysate.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4

Concentration

Concentration information loading...

Purity

IgG fraction

Purification notes

The antiserum was fractionated and further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.

Clonality

Polyclonal

Isotype

IgG

Research areas

Cell Biology >> Other Antibodies >> Other Antibodies
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKC
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Cdk Regulators

  • Western blot - RACK1 antibody (ab62735)Western blot - RACK1 antibody (ab62735) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab62735 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Seems to bind protein kinase C acting as an intracellular receptor to anchor the activated PKC to the cytoskeleton. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and ITGB1, serves as a platform for SRC activation or inactivation. May play an important role in the developing brain and neuronal differentiation.

Sequence similarities

Belongs to the WD repeat G protein beta family.
Contains 7 WD repeats.

Domain

The WD repeats domain 5 mediates interaction with TRIM63.

Cellular localization

Cell membrane. Located on plasma membrane of neuroblastoma NMB7 cells.

Target information above from: UniProt accessionP63244 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Cell proliferation-inducing gene 21 protein antibody
  • GBLP_HUMAN antibody
  • Gnb2 rs1 antibody
  • Gnb2-rs1 antibody
  • GNB2L1 antibody
  • Gnb2rs1 antibody
  • Guanine nucleotide binding protein (G protein) beta polypeptide 2 like 1 antibody
  • Guanine nucleotide binding protein beta polypeptide 2 like 1 antibody
  • Guanine nucleotide binding protein beta subunit 2 like 1 antibody
  • Guanine nucleotide binding protein beta subunit like protein 12.3 antibody
  • Guanine nucleotide binding protein subunit beta 2 like 1 antibody
  • Guanine nucleotide binding protein subunit beta like protein 12.3 antibody
  • Guanine nucleotide-binding protein subunit beta-2-like 1 antibody
  • Guanine nucleotide-binding protein subunit beta-like protein 12.3 antibody
  • H12.3 antibody
  • HLC 7 antibody
  • HLC-7 antibody
  • HLC7 antibody
  • Human lung cancer oncogene 7 protein antibody
  • Lung cancer oncogene 7 antibody
  • OTTHUMP00000223704 antibody
  • OTTHUMP00000223870 antibody
  • OTTHUMP00000223891 antibody
  • OTTHUMP00000223893 antibody
  • OTTHUMP00000223900 antibody
  • OTTHUMP00000223902 antibody
  • OTTHUMP00000223930 antibody
  • OTTHUMP00000223931 antibody
  • PIG 21 antibody
  • PIG21 antibody
  • Proliferation inducing gene 21 antibody
  • Protein homologous to chicken B complex protein guanine nucleotide binding antibody
  • RACK 1 antibody
  • RACK1 antibody
  • Receptor for activated C kinase antibody
  • Receptor of activated protein kinase C 1 antibody
see all

Database links

see all

Anti-RACK1 antibody images:

  Western blot - RACK1 antibody (ab62735)

Western blot - RACK1 antibody (ab62735)

All lanes : Anti-RACK1 antibody (ab62735) at 1/500 dilution

Lane 1 : Extract from hek-293T cell line
Lane 2 : Extract from HeLa cell line
Lane 3 : Extract from MCF7 cell line
Lane 4 : Extract from U2OS cell line
Lane 5 : Extract from Jurkat cell line
Lane 6 : Extract from RAT1 cell line
Lane 7 : Extract from PC12 cell line
Lane 8 : Extract from NIH3T3 cell line

Secondary
Goat Anti-Rabbit IgG
developed using the ECL technique

Predicted band size : 35 kDa
Observed band size : 30 kDa (why is the actual band size different from the predicted?)

References for Anti-RACK1 antibody (ab62735)

This product has been referenced in:

  • Zheng Yet al. Interaction of protein inhibitor of activated STAT 2 (PIAS2) with receptor of activated C kinase 1, RACK1. FEBS Lett 586:122-6 (2012). ICC/IF, IP.Read more (PubMed: 22210188) »

See 1 publication for this product

Publishing research using ab62735? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"