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All lanes : Anti-RACK1 antibody (ab62736) at 1 µg/ml
Lane 1 : Extract from HEK-293T cell line
Lane 2 : Extract from HeLa cell line
Lane 3 : Extract from MCF7 cell line
Lane 4 : Extract from U2OS cell line
Lane 5 : Extract from Jurkat cell line
Lane 6 : Extract from RAT1 cell line
Lane 7 : Extract from PC12 cell line
Lane 8 : Extract from NIH3T3 cell line
Secondary
Goat Anti-Rabbit IgG
developed using the ECL technique
Predicted band size : 35 kDa
Observed band size : 30 kDa (why is the actual band size different from the predicted?)
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