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Products:Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Cdk Regulators
Overview
Product name
Anti-RACK1 antibody
See all RACK1 products (8) ...
Description
Rabbit polyclonal to RACK1
Tested applications
WB, IPmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
a wide range of other species
Immunogen
Synthetic peptide: C-DEL KQEVISTSSK AE conjugated to KLH by an N-terminal Cysteine residue linker, corresponding to near C-terminal amino acids 268-282 of Human RACK1.
C-DEL KQEVISTSSK AE
Positive control
HEK-293T cell lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Other Antibodies >> Other Antibodies
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKC
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Cdk Regulators
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Western blot - RACK1 antibody (ab62736)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab62736 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
IP
Application notes
IP: Use at 5-10 µg.
WB: Use at a concentration of 0.5 - 1.0 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 35 kDa).
Staining of the RACK1 band is specifically inhibited by the immunizing peptide.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Seems to bind protein kinase C acting as an intracellular receptor to anchor the activated PKC to the cytoskeleton. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and ITGB1, serves as a platform for SRC activation or inactivation. May play an important role in the developing brain and neuronal differentiation.
Sequence similarities
Belongs to the WD repeat G protein beta family.
Contains 7 WD repeats.
Domain
The WD repeats domain 5 mediates interaction with TRIM63.
Cellular localization
Cell membrane. Located on plasma membrane of neuroblastoma NMB7 cells.
Target information above from: UniProt accessionP63244
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Cell proliferation-inducing gene 21 protein antibody
- GBLP_HUMAN antibody
- Gnb2 rs1 antibody
see all
Database links
- Entrez Gene: 10399 Human
- Entrez Gene: 14694 Mouse
- Entrez Gene: 83427 Rat
- Omim: 176981 Human
- SwissProt: P63244 Human
- SwissProt: P68040 Mouse
- SwissProt: P63245 Rat
- Unigene: 5662 Human
see all
Anti-RACK1 antibody images:
Western blot - RACK1 antibody (ab62736)
All lanes : Anti-RACK1 antibody (ab62736) at 1 µg/ml
Lane 1 : Extract from HEK-293T cell line
Lane 2 : Extract from HeLa cell line
Lane 3 : Extract from MCF7 cell line
Lane 4 : Extract from U2OS cell line
Lane 5 : Extract from Jurkat cell line
Lane 6 : Extract from RAT1 cell line
Lane 7 : Extract from PC12 cell line
Lane 8 : Extract from NIH3T3 cell line
Secondary
Goat Anti-Rabbit IgG
developed using the ECL technique
Predicted band size : 35 kDa
Observed band size : 30 kDa (why is the actual band size different from the predicted?)
References for Anti-RACK1 antibody (ab62736)
ab62736 has not yet been referenced specifically in any publications.
Publishing research using ab62736? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"