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RAD18 293T Transfected Lysate - (positive control) (ab94312)

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Overview

  • Product nameRAD18 293T Transfected Lysate - (positive control)
  • General notesab94312 is a 293T cell transfected lysate in which Human RAD18 has been transiently over-expressed using a pCMV-RAD18 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
  • Tested applicationsWB more details

Properties

  • BackgroundFunction: E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity. Pathway: Protein modification; protein ubiquitination. Similarity: Belongs to the RAD18 family. Contains 1 RING-type zinc finger. Contains 1 SAP domain. Contains 1 UBZ-type zinc finger.

    • Applications

    Our Abpromise guarantee covers the use of ab94312 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB
  • Application notesWB: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • RAD18 293T Transfected Lysate - (positive control) images

    • SDS-PAGE - RAD18 293T Transfected Lysate - (positive control) (ab94312)
      SDS-PAGE - RAD18 293T Transfected Lysate - (positive control) (ab94312)
      ab94312 at 15µg/lane on an SDS-PAGE gel
    • Western blot - RAD18 293T Transfected Lysate - (positive control) (ab94312)
      Western blot - RAD18 293T Transfected Lysate - (positive control) (ab94312)
      All lanes : Anti-RAD18 antibody (ab57447) at 1/500 dilution

      Lane 1 : RAD18 293T Transfected Lysate - (positive control) (ab94312)
      Lane 2 : 293T non-transfected lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

    References for RAD18 293T Transfected Lysate - (positive control) (ab94312)

    ab94312 has not yet been referenced specifically in any publications.

    Publishing research using ab94312 ? Please let us know so that we can cite the reference in this datasheet

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"