Anti-RAD18 antibody (ab79763)
Key features and details
- Rabbit polyclonal to RAD18
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-RAD18 antibody
See all RAD18 primary antibodies -
Description
Rabbit polyclonal to RAD18 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Does not react with: Mouse -
Immunogen
Fusion protein corresponding to Human RAD18 (C terminal). 113 carboxyl terminal of human Rad18
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Positive control
- A549 cells GM637 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab79763 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Predicted molecular weight: 56 kDa.
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ICC/IF |
Use at an assay dependent concentration.
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Notes |
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WB
1/1000. Predicted molecular weight: 56 kDa. |
ICC/IF
Use at an assay dependent concentration. |
Target
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Function
E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the RAD18 family.
Contains 1 RING-type zinc finger.
Contains 1 SAP domain.
Contains 1 UBZ-type zinc finger. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 56852 Human
- Omim: 605256 Human
- SwissProt: Q9NS91 Human
- Unigene: 375684 Human
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Alternative names
- 2810024C04Rik antibody
- DNA repair protein rad18 antibody
- E3 ubiquitin-protein ligase RAD18 antibody
see all
Images
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Indirect immunofluorescence staining of GM637 cells . Note staining of nuclei.
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Anti-RAD18 antibody (ab79763) at 1/1000 dilution + A549 crude extract
Predicted band size: 56 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands.
The lower thick band is native RAD18 and the upper thin band is mono-ubiquitinated RAD18 protein.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab79763 has been referenced in 5 publications.
- Wanzel M et al. CRISPR-Cas9-based target validation for p53-reactivating model compounds. Nat Chem Biol 12:22-8 (2016). WB . PubMed: 26595461
- Peng M et al. Crosstalk between BRCA-Fanconi anemia and mismatch repair pathways prevents MSH2-dependent aberrant DNA damage responses. EMBO J N/A:N/A (2014). WB ; Human . PubMed: 24966277
- Zeman MK et al. DNA damage-specific deubiquitination regulates Rad18 functions to suppress mutagenesis. J Cell Biol 206:183-97 (2014). Human . PubMed: 25023518
- Jones MJ et al. Dysregulation of DNA polymerase ? recruitment to replication forks results in genomic instability. EMBO J 31:908-18 (2012). WB ; Human . PubMed: 22157819
- Cotto-Rios XM et al. APC/CCdh1-dependent proteolysis of USP1 regulates the response to UV-mediated DNA damage. J Cell Biol 194:177-86 (2011). WB ; Human . PubMed: 21768287